›› 2011, Vol. 23 ›› Issue (2): 134-136.doi: 10.3969/j.issn.1004-616x.2011.02.012

• 技术与方法 • Previous Articles     Next Articles

The value of G-banding chromosomal assay in ionizing-radiation-induced chromosomal aberration

SHI Chang-bei1，CHEN Xiao-quan1，YAO Jun-tao1，YUAN Yong1，ZHAO Zheng1，YUAN Bin1，ZHAO Minggang2，CHEN Wei，WANG Xiang2,*   

  1. 1. Shaanxi Tumor Hospital, Xi’an 710061，Shaanxi; 2. First Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710061, Shaanxi, China
  • Received:2010-12-12 Revised:2011-01-12 Online:2011-03-30 Published:2011-03-30
  • Contact: WANG Xiang

PDF (PC)

1818

Abstract: To validate the value of G-banding chromosomal assay in ionizing-radiation-induced chromosomal aberration. METHODS: Blood was irradiated with 0.5，1， 2 and 4 Gy，by 4 MV X-ray. The G-banding chromosomal aberration was assayed including dicentrics， deletions， translocations， rings， and fragments of chromosomes. RESULTS: Chromosomal aberration rates of dicentrics， deletions， translocations， and fragments all showed linear increase with radiation dose，with correlations of Y=24.1X-13.3(R2=0.975)；Y=10.5X-2.7(R2=0.887)； Y=30.2X-17.8(R2=0.913)；Y=53.3X-38.7 (R2=0.976)，respectively. CONCLUSION: G-banding chromosomal aberration assay deserve more attention in radiation biology.

Key words: radiation, chromosomal aberration assay, G-banding technique