ERK和SAPK/JNK通路在神经酰胺致HT-29细胞凋亡中的作用

doi:10.3969/j.issn.1004616x.2012.06.003

Abstract

Abstract:

OBJECTIVE: To study the mechanisms of ERK and SAPK/JNK pathways of ceramide-induced apoptosis in HT-29 cells. METHODS：HT-29 cells were treated with C2-ceramide for 24 hours at different doses (0，12.5，25.0，50.0μmol/L). AO/EB fluorescent staining was used to identify the morphological changes of HT-29 cells and evaluate the rate of apoptosis in 200 cells. The protein expressions of ERK and phosphorylated ERK(p-ERK) were evaluated by Western Blotting. The activity of phospho-c-Jun in different doses and treatment time of C2-ceramide groups was assessed using SAPK/JNK assay and Western Blotting. RESULTS：Apoptosis rate was increased and the expressions of ERK and p-ERK were reduced in a dose-dependent manner (P＜0.05). The activity of phospho-c-jun in different doses and treatment time showed no obvious changes (P＜0.05). CONCLUSION：C2-ceramide could directly induce apoptosis in human colon carcinoma HT-29 cells in vitro，and C2-ceramide could inhibit the ERK pathway without activating SAPK/JNK pathway during this process.

Key words: ceramide, poptosis, APK/JNK pathway, RK pathway

BAI Yan-yan1，2，WU Yan-ping2，ZHANG Xiao-feng2，LI Bai-xiang2，*. Mechanisms of ERK and SAPK/JNK pathways of ceramide-induced apoptosis in HT-29 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004616x.2012.06.003.

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Mechanisms of ERK and SAPK/JNK pathways of ceramide-induced apoptosis in HT-29 cells

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