Abstract:

 OBJECTIVE: To explore the cytotoxic effects of 9 different solvents with different volume fractions on CHL cells in vitro. METHODS：MTT was used to study the inhibition rates of different solvents on CHL cells at 0.1%，0.3%，1%，3%，10% volume fractions，and thus to calculate the volume fraction of each solvent causing cell inhibition rate of 20%. RESULTS：When the inhibition rates of DMSO，diethylene dioxide，acetonitrile， acetone， tetrahydrofuran，glycerine，acetic acid，methanol and ethanol on CHL cells reached 20%，the volume fractions were 1.0%，0.2%，1.5%，5.4%，0.2%，3.2%，0.000 07%，1.7% and 2.1%，respectively. CONCLUSION：When performing chromosome aberration test，one should first fully understand the characteristics of the test substance，on this basis， to select the appropriate solvent according to their characteristics and toxicities.

Key words: chromosome aberration test, solvent, CHL cell, cytotoxicity