Hoechst33342/PI双染法和TUNEL染色技术检测神经细胞凋亡的对比研究

doi:10.3969/j.issn.1004-616x.2014.03.005

Abstract

Abstract:

OBJECTIVE: To evaluate the effects of nano-silica(nm-SiO2) on apoptosis of neuroblastoma cells，and compare the advantages and disadvantages of Hoechst33342/PI staining and TUNEL assay in apoptosis measurement. METHODS：Cultured human neuroblastoma SK-N-SH cells were treated with nm-SiO2 (15 and 30 nm) and micro-sized SiO2 at different doses (2.5，5 and 10 μg/mL) for 24 h. Apoptotic cells were detected by Hoechst33342/PI and TUNEL staining. RESULTS：Compared with normal control，the percentages of total apoptosis in nm-SiO2-treated cells were significantly increased by both methods. CONCLUSION：nm-SiO2 increased apoptosis of SK-N-SH cells in a dose- and size-dependent manner. Hoechst33342/PI is more specific，cheaper and simpler，while TUNEL assay is more sensitive. It is recommended to combine these two methods to assess apoptosis in neuronal cells.

Key words: nm-SiO2, neuronal cells, apoptosis, Hoechst33342/PI double staining, TUNEL

YANG Xi-fei，HE Chun-e，TANG Rui-hua，TIAN Sheng-li，LIU Jian-jun，. A comparative study on the measurement of neuronal cell apoptosis by Hoechst33342/PI double staining and TUNEL assay[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.03.005.

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A comparative study on the measurement of neuronal cell apoptosis by Hoechst33342/PI double staining and TUNEL assay

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