去甲斑蝥素抑制大鼠心肌细胞缺血再灌注损伤的研究

doi:10.3969/j.issn.1004-616x.2014.06.005

Abstract

Abstract:

OBJECTIVE: To explore the effects of norcantharidin (NCTD) on myocardial ischemia-reperfusion injury (IRI) and its possible mechanisms in rats. METHODS：The effect of different concentrations of NCTD on primary rat cardiomyocytes and H9c2 cells was examined by MTT assay. The apoptosis effect of NCTD pretreatment on IRI is detected by flow cytometry. Ischemia-reperfusion myocardial cell morphology of NCTD pretreatment was examined by Giemsa and Trypan blue staining. The expression of programmed cell death factor 4 (Pdcd4) in NCTD-pretreated ischemia-reperfusion myocardial cell was examined by Western blot. The expression of microRNA-21(miR-21) in NCTD-pretreated ischemia-reperfusion myocardial cell was assessed by RT-qPCR. RESULTS：The IRI of primary rat cardiomyocytes and H9c2 was inhibited by NCTD (P<0.01). Cell death caused by IRI was significantly inhibited by NCTD. NCTD could effectively inhibit Pdcd4 protein up-regulation and miR-21 down-regulation caused by IRI in rat cardiomyocytes. CONCLUSION：Myocardial IRI could be inhibited by NCTD treatment in concentration of 0.1-0.5 μg/mL，and miR-21-Pdcd4 signaling pathways may participate in myocardial IRI and the inhibition of IRI by NCTD.

Key words: norcantharidin, rat , cardiac , myocyte, myocardial , ischemia- reperfusion , injury, programmed , cell , death , 4, microRNA-21

CUI Bao-di，WU Yang#，SUN Zhen-xiao*. Protective effect of norcantharidin on ischemia-reperfusion injury in rat cardiomyocytes[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.06.005.

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Protective effect of norcantharidin on ischemia-reperfusion injury in rat cardiomyocytes

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