异欧前胡素对人表皮黑素细胞酪氨酸酶和Rab27a的调节作用

doi:10.3969/j.issn.1004-616x.2018.01.007

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2018, Vol. 30 ›› Issue (1): 37-41.doi: 10.3969/j.issn.1004-616x.2018.01.007

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Effects of isoimperaterin on regulation of tyrosinase and Rab27a in human epidermal melanocytes

SUN Beilei¹, ZHENG Yuqiong², LIN Ling¹, CHEN Weiying¹, CAI Erhui³, CHEN Weifang³

1. Experimental Teaching Center for Preclinical Medicine, Shantou University Medical College, Shantou 515041;
2. The First Affiliated Hospital, Shantou University Medical College, Shantou 515041;
3. The Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2017-09-26 Revised:2017-12-08 Online:2018-01-30 Published:2018-01-30

Abstract

Abstract: OBJECTIVE: To investigate the effects of isoimperaterin on regulation of tyrosinase and Rab27a in cultured human epidermal melanocytes. METHODS: Human epidermal melanocytes were isolated and cultured. The cells were randomly divided into control and experimental groups. Melanocytes of the control group were cultured with M-254 culture medium. Cells of the experimental group were cultured with M-254 culture medium containing 25 μmol/L isoimperaterin. The cells were cultured for 48 h and observed using inverted microscopy. Cell vitality was assayed using MTT method. The expression of tyrosinase and transfer-related protein Rab27a were analyzed by western blotting. The activity of tyrosinase and the content of melanin were measured by L-dopa oxidation and NaOH method,respectively. RESULTS: Compared with the control group,the dendrites of melanocytes of experimental group were elongated after treatment with 25 μmol/L isoimperaterin for 48 h. Melanocyte vitality was reduced by 17.2% (P < 0.05). Expression of tyrosinase was decreased by 30.3% (P < 0.01) and Rab27a was increased by 31.9% (P < 0.05). The activity of tyrosinase was reduced by 25.6% (P < 0.05). Melanin content was decreased by 30.9% (P < 0.01). CONCLUSION: 25 μmol/L isoimperaterin elongated melanocytic dendrites but inhibited melanocyte vitality. It also inhibited the expression and activity of tyrosinase and promoted the expression of transfer-related protein Rab27a to reduce the content of melanin in cultured human epidermal melanocytes.

Key words: melanocyte, isoimperatorin, tyrosinase, Rab27a, melanin

CLC Number:

R329.2

SUN Beilei, ZHENG Yuqiong, LIN Ling, CHEN Weiying, CAI Erhui, CHEN Weifang. Effects of isoimperaterin on regulation of tyrosinase and Rab27a in human epidermal melanocytes[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 37-41.

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Effects of isoimperaterin on regulation of tyrosinase and Rab27a in human epidermal melanocytes

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[1]	ZHAN Biao, SUN Beilei, LUO Sicong, ZHUANG Hongkai, HUANG Pengzhou, ZENG Yi, CHEN Weifang, CHEN Weiying. Regulatory effects of isoimperatorin on expression of tyrosinase and β-actin in cultured human epidermal melanocytes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 364-369.
[2]	XIE Jia-jun，LIN Ling，LIU Shao-gang，WU Xiao-jia，RUAN Hai-dong，GUO Pei-xiang，TONG Peng-fei，FANG Bin，CHEN Wei-ying，SHE Shao-yi. Effects of imperatorin on Rab27a and tyrosinase in cultured human epidermal melanocytes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2014, 26(4): 279-284.