DKK-1和NNMT与肝癌患者临床病理指标及预后的相关性

doi:10.3969/j.issn.1004-616x.2024.06.005

Abstract

Abstract: OBJECTIVE： To investigate the involvement of Dickkopf-related protein-1 (DKK-1) and nicotinamide N-methyltransferase (NNMT) in clinicopathology and prognosis among liver cancer patients. METHODS： Expression levels of NNMT and DKK-1 mRNA in normal liver tissues and liver cancer cells were analyzed by GEPIA2 database.，Effects of the two on the overall survival (OS) and disease-free survival (DFS) of patients with liver cancer were analyzed. Immunohistochemistry was used to detect the protein expression of NNMT and DKK-1 in liver tumor group，benign lesion group and normal group，and the relationship between them and the occurrence and development of liver cancer and clinicopathological features was analyzed. RESULTS： The GEPIA2 database showed that expression levels of NNMT mRNA in liver cancer tissues were significantly lower than that in normal liver tissues (P<0.05). Expressions of NNMT and DKK-1 mRNA in liver cancers were negatively correlated (r=-0.13，P<0.05). Both had no significant effect on the 10-year OS and DFS of liver cancer patients. The 5-year survival rate of liver cancer patients with high expression of NNMT mRNA was higher than that of those with low expression (P<0.05). The results of immunohistochemistry showed that compared with normal liver tissues，the expressions of DKK-1 and NNMT proteins in liver cancer tissues were significantly reduced (P<0.01). Expression of DKK-1 protein in hepatocellular carcinoma was significantly higher than that in cholangiocarcinoma. The expression of both proteins was not significantly correlated with tumor size，stage and tissue grade (P>0.05). CONCLUSION： The expression levels of DKK-1 and NNMT proteins in liver cancer tissues are lower than those in normal liver tissues. The expression levels of DKK-1 protein were related to the tissue types of liver cancers，and the expression levels of NNMT mRNA affected the 5-year survival rate of patients with liver cancer.

Key words: liver cancer, NNMT, DKK-1, clinicopathological features, survival rate

CLC Number:

R735.7

WU Xiuqian, LIANG Tairong, ZHUANG Xuelong, WANG Xingxing, HUANG Haihua. Involvement of DKK-1 and NNMT in clinicopathology and prognosis among liver cancer patients[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2024, 36(6): 452-456,462.

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Involvement of DKK-1 and NNMT in clinicopathology and prognosis among liver cancer patients

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