放射性皮肤损伤中细胞干性的转变

doi:10.3969/j.issn.1004-616x.2026.02.004

Abstract

Abstract: OBJECTIVE: To investigate changes in cellular stemness transition in radiation-induced skin injury in cell culture. METHODS: Human immortalized keratinocytes(Ha Ca T) were exposed to X-ray at 0,5,10,and 20 Gy. Cell migration ability at 24 h and 48 h post-irradiation was assessed using the scratch wound healing assay and transwell migration assay. Western blot was used to detect the expression of stemness-related proteins KLF4,c-myc and LGR5 in Ha Ca T cells at 24 h and 48 h after irradiation. In the animal experiments,the skin of the right hind limb of SD rats was irradiated with a single dose of 20 Gy X-ray. At 15 and 35 days post-irradiation, western blot was performed to detect the expression of stemness-related proteins SOX2,Nanog, KLF4, c-myc, and LGR5 in the irradiated skin cells. A non-irradiated control group(0 Gy) was included, with 4 rats per group. RESULTS: Compared with the control group(0 Gy), the in vitro scratch wound healing assay at 48 h post-irradiation showed that cell migration ability was significantly reduced in the5, 10, and 20 Gy groups(P<0.05). In contrast, the transwell assay demonstrated significantly enhanced migration ability in all irradiated groups(P<0.01), with the 10 Gy group still retaining a certain level of migration capacity. At 24 h post-irradiation,KLF4,c-myc and LGR5 protein expression levels in Ha Ca T cells were significantly decreased in all irradiated dose groups(P<0.01). At 48 h post-irradiation,KLF4,c-myc and LGR5 protein expression levels were significantly increased(P<0.01). In the animal experiments,compared with the control group(0 Gy), at 15 days after 20 Gy X-ray irradiation, expression levels of stemness-related proteins SOX2, c-Myc, and LGR5 in rat skin were significantly elevated(all P<0.01). At 35 days postirradiation, expression levels of SOX2, Nanog, and LGR5 were significantly decreased(P<0.05 or P<0.01).CONCLUSION: In radiation-induced skin injury, cellular stemness transition capacity expressed dynamic changes which might cause inhibition of repair,promotion of regeneration with gradual decline in the long term.

Key words: ionizing radiation, skin injury, stemness genes, repair

CLC Number:

R994.6

GUAN Ziyi, YU Nanxi, CHENG Xueyan, MA Liping, GAO Ling. Cellular stemness transition in radiation-induced skin injury in vitro and in vivo[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2026, 38(2): 108-112,118.

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Cellular stemness transition in radiation-induced skin injury in vitro and in vivo

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