氯化铬和重铬酸钾毒性的比较研究

doi:10.3969/j.issn.1004-616x.2003.03.013

癌变·畸变·突变 ›› 2003, Vol. 15 ›› Issue (3): 171-173.doi: 10.3969/j.issn.1004-616x.2003.03.013

氯化铬和重铬酸钾毒性的比较研究

马华智，盛和章，秦爱平，施　畅，李永涛，廖明阳

军事医学科学院毒物药物研究所六所四室，北京　100850

收稿日期:2002-07-10 修回日期:2002-11-13 出版日期:2003-07-30 发布日期:2003-07-30
通讯作者: 廖明阳

COMPARATIVE STUDY ON TOXICITIES OF POTASSIUM DICHROMATE AND CHROMIUM CHLORIDE

MA Hua-zhi, SHENG He-zhang, QIN Ai-ping,et al

Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences,Beijing, 100850, China

Received:2002-07-10 Revised:2002-11-13 Online:2003-07-30 Published:2003-07-30
Contact: LIAO Ming-yang

摘要/Abstract

摘要： 目的：比较氯化铬和重铬酸钾两种价态铬化合物的细胞毒性、致突变性及急性毒性。方法： CHL细胞采用MTT比色法，沙门氏菌诱变性试验和小鼠急性毒性试验。结果：经MTT比色法测定，氯化铬和重铬酸钾对CHL细胞半数抑制剂量(IC 50)分别为33.80 mmol / L和8.02 μ mol / L，对小鼠口服急性毒性LD 50分别为2 143.3 和171.0 mg / kg，表明重铬酸钾的细胞毒性和急性毒性均明显大于氯化铬，重铬酸钾诱发沙门氏菌回变菌落数比阴性对照增加2倍以上而氯化铬无明显增加。结论：重铬酸钾的细胞毒性和急性毒性均大于氯化铬，重铬酸钾对沙门氏菌具有致突变性，而氯化铬无致突变性。

关键词: 重铬酸钾, 氯化铬, MTT, 沙门氏菌诱变性试验

Abstract: Purpose:To compare the cycotoxixity,mutagenncity and mouse acute toxicity between Chromium chloride and Potassium dichromate. Methods: MTT method for determition of CHL Cell IC 50, Salmonella mutagenicity test, and mouse acute toxicity test were used. Results: The CHL cell IC 50 of chromium chloride and potassium dichromate were 33.80 mmol / L and 8.02 μmol / L,respectively; the LD 50 of chromium chloride and potassium dichromate in mouse acute toxicity test were 2 143.3 and 171.0 mg / kg,respectively. Potassium dichromate meet the positive respond standards of Salmonella mutagenicity test whereas chromium chloride showed a negative results. Conclusion: Both the cytotoxicity and mouse acute toxicity of potassium dichromate was larger than chromium chloride. Potassium dichromate was mutagennic to Salomamella strains,but chromium chloride was not.

Key words: potassium dichromate, chromium chloride, MTT, Slamonella mutagenicity test

马华智, 盛和章, 秦爱平, 施　畅, 李永涛, 廖明阳. 氯化铬和重铬酸钾毒性的比较研究[J]. 癌变·畸变·突变, 2003, 15(3): 171-173.

MA Hua-zhi, SHENG He-zhang, QIN Ai-ping, et al. COMPARATIVE STUDY ON TOXICITIES OF POTASSIUM DICHROMATE AND CHROMIUM CHLORIDE[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2003, 15(3): 171-173.

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氯化铬和重铬酸钾毒性的比较研究

COMPARATIVE STUDY ON TOXICITIES OF POTASSIUM DICHROMATE AND CHROMIUM CHLORIDE

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