人骨髓间充质干细胞在新生小鼠脑中的植入和分化

doi:10.3969/j.issn.1004-616x.2007.01.002

癌变·畸变·突变 ›› 2007, Vol. 19 ›› Issue (1): 4-007.doi: 10.3969/j.issn.1004-616x.2007.01.002

人骨髓间充质干细胞在新生小鼠脑中的植入和分化

陶欣荣1,2；李文林1；苏　娟1；王新民1；李建秀1；胡以平1,

1. 第二军医大学基础部细胞生物学教研室，上海 200433；2. 安徽理工大学医学院，安徽淮南 232001

收稿日期:2006-08-12 修回日期:2006-09-18 出版日期:2007-01-30 发布日期:2007-01-30

Engraftment and Differentiation of Human Bone Marrow Mesenchymal Stem Cells in Neonatal Mouse Brain

TAO Xin-rong1,2, LI Wen-lin1, SU Juan1, WANG Xin-min1, LI Jian-xiu1, HU Yi-ping1,

1. Department of Cell Biology, Second Military Medical University, Shanghai 200433, China; 2．Medical College，Anhui University of Science and Technology, Huainan 232001, China)

Received:2006-08-12 Revised:2006-09-18 Online:2007-01-30 Published:2007-01-30

摘要/Abstract

摘要： 背景与目的：探讨人骨髓间充质干细胞(human mesenchymal stem cells, hMSCs)在体内向神经细胞分化的可能性。材料与方法：将标记绿色荧光蛋白的人骨髓间充质干细胞植入到新生3 d的小鼠侧脑室中，分别于移植后0 d，9 d和14 d处死受体鼠，取其脑组织，4％多聚甲醛固定后行冠状面冰冻切片，荧光显微镜下观察hMSCs的植入，激光共聚焦显微镜检测植入细胞神经特异性蛋白的表达，定位双重标记的植入细胞。结果：受体小鼠脑中均可检出植入的细胞，此类细胞表达神经元细胞特异的微管相关蛋白β-Ⅲ-Tubulin (Tuj1)、微管相关蛋白2(MAP2)，一些细胞表达神经胶质细胞特异的胶质原纤维酸性蛋白(GFAP)。结论： hMSCs植入后受到脑组织特定微环境的影响，在体内可以向神经细胞分化并参与到发育的神经系统中。

关键词: 人骨髓间充质干细胞, 细胞移植, 分化, 神经细胞

Abstract: BACKGROUND ＆ AIM:To explore the possibility of human mesenchymal stem cells (hMSCs) differentiating into neural cells in vivo. MATERIALS AND METHODS: hMSCs labeled with enhanced green fluorescent protein (EGFP) were transplanted into the lateral ventricle of neonatal mouse brain. At 0, 9 and 14 days post-transplantation, mice were sacrificed and their brains were fixed with 4％ paraformaldehyde. The engraftment of transplanted cells in coronal section of the recipient mouse brain was examined under a fluorescent microscope. Indirected immunofluorescent staining was used to detect the expression of neural proteins of these grafted cells. RESULTS: Examination of cryostat section under fluorescent microscopy revealed that hMSCs could engraft into neonatal mouse brain. Neuronal differentiation was confirmed by the expression of β-Ⅲ-Tubulin (Tuj1) and MAP2 of these EGFP-expressing cells, whilst some grafted cells had GFAP staining. CONCLUSION: These results suggest that hMSCs could respond to brain microenvironment and differentiate into neural cells.

Key words: human mesenchymal stem cells, cell transplantation, differentiation, neural cells

中图分类号:

Q254

陶欣荣, 李文林, 苏　娟, 王新民, 李建秀, 胡以平, . 人骨髓间充质干细胞在新生小鼠脑中的植入和分化[J]. 癌变·畸变·突变, 2007, 19(1): 4-007.

TAO Xin-rong, LI Wen-lin, SU Juan, WANG Xin-min, LI Jian-xiu, HU Yi-ping, . Engraftment and Differentiation of Human Bone Marrow Mesenchymal Stem Cells in Neonatal Mouse Brain[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2007, 19(1): 4-007.

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人骨髓间充质干细胞在新生小鼠脑中的植入和分化

Engraftment and Differentiation of Human Bone Marrow Mesenchymal Stem Cells in Neonatal Mouse Brain

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