瑞-姬氏混染在Cr(Ⅵ)诱导的HepG2细胞微核试验中的应用

doi:10.3969/j.issn.1004-616x.2007.02.019

癌变·畸变·突变 ›› 2007, Vol. 19 ›› Issue (2): 146-148.doi: 10.3969/j.issn.1004-616x.2007.02.019

瑞-姬氏混染在Cr(Ⅵ)诱导的HepG2细胞微核试验中的应用

边寰锋安飞云钟才高廖春华

1.中南大学公共卫生学院卫生毒理学系，湖南　长沙　410078；2.江西省疾病预防控制中心，江西　南昌　330029

收稿日期:2006-06-13 修回日期:2006-10-30 出版日期:2007-03-30 发布日期:2007-03-30
通讯作者: 钟才高

Utilization of Wright-Giemsa Mixed Stain in Cr(Ⅵ) –Induced HepG2 Cells Micronuclei Test

BIAN Huan-feng1， AN Fei-yun1，ZHONG Cai-gao1,， LIAO Chun-hua2

1.Department of Health Toxicology, School of Public Health, Central South University， Changsha 410078, China; 2. Jiangxi Center for Disease Prevention and Control, Nanchang 330029, China

Received:2006-06-13 Revised:2006-10-30 Online:2007-03-30 Published:2007-03-30
Contact: ZHONG Cai-gao

摘要/Abstract

摘要： 背景与目的：将瑞-姬(Wright-Giemsa)氏混染法用于Cr(Ⅵ)诱导的HepG2细胞微核试验，探讨瑞-姬氏混染配比浓度和不同染色时间对染色效果的影响。材料与方法：以瑞-姬氏混染对HepG2细胞进行染色，采用不同的染液配比和染色时间，观察其对染色效果的影响。结果: 3∶1瑞-姬氏混染3～5 min可以获得较瑞氏或姬氏单一染色更好的染色效果。结论: 瑞-姬氏混合染色法可应用于微核试验中的细胞染色，并可获得良好的染色效果。

关键词: 瑞氏染色, 姬氏染色, 瑞-姬氏混染, 双核, HepG2细胞

Abstract: BACKGROUND ＆ AIM: To explore the influence of different concentrations and different proportions of Wright-Giemsa dye on its staining effect, through application to micronucleus test of HepG2 cells induced by hexavalent chromium[Cr(Ⅵ)]. MATERIALS AND METHODS: HepG2 cells were stained with different proportions of Wright-Giemsa dye and for different stain time, and their influence on staining effects were assessed. RESULTS: Better results could be obtained when cells were stained with a concentration of Wright-Giemsa at 3∶1 for 3-5 minutes. CONCLUSION: Wright-Giemsa stain could be applied to micronucleus test with favorable effects.

Key words: Wright stain, Giemsa stain, Wright-Giemsa stain, binuclei, HepG2 cells

中图分类号:

Q355

边寰锋安飞云钟才高廖春华. 瑞-姬氏混染在Cr(Ⅵ)诱导的HepG2细胞微核试验中的应用[J]. 癌变·畸变·突变, 2007, 19(2): 146-148.

BIAN Huan-feng, AN Fei-yun, ZHONG Cai-gao, , LIAO Chun-hua. Utilization of Wright-Giemsa Mixed Stain in Cr(Ⅵ) –Induced HepG2 Cells Micronuclei Test[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2007, 19(2): 146-148.

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瑞-姬氏混染在Cr(Ⅵ)诱导的HepG2细胞微核试验中的应用

Utilization of Wright-Giemsa Mixed Stain in Cr(Ⅵ) –Induced HepG2 Cells Micronuclei Test

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