广西黄曲霉毒素B1高污染区210人CYP3A7基因多态性检测

doi:10.3969/j.issn.1004-616x.2008.04.011

癌变·畸变·突变 ›› 2008, Vol. 20 ›› Issue (4): 292-294.doi: 10.3969/j.issn.1004-616x.2008.04.011

广西黄曲霉毒素B1高污染区210人CYP3A7基因多态性检测

陆海霞/ 冯震博/冯小兰

广西医科大学病理学教研室，南宁　530021

收稿日期:2007-11-09 修回日期:2008-03-19 出版日期:2008-07-30 发布日期:2008-07-30

Polymorphisms of CYP3A7 Gene of 210 Persons in Guangxi with Heavy Aflatoxin B1 Contamination

LU Hai_xia,FENG Zhen_bo,FENG Xiao_lan

The Department of Pathology,Guangxi Medical University,Nanning 530021，China

Received:2007-11-09 Revised:2008-03-19 Online:2008-07-30 Published:2008-07-30

摘要/Abstract

摘要： 背景与目的：了解广西黄曲霉毒素B1(AFB1)高污染区人群细胞色素P450 3A7(CYP3A7)基因的多态性，及其基因型与肝细胞癌易感性之间的关系。材料与方法：选取黄曲霉毒素B1高污染区人群210例，其中肝细胞性肝癌患者97例，设为肝细胞性肝癌组(HCC组)；HBV阳性而无其它疾病者38例，设为HBV阳性组； HBV阴性且无疾病者75例，设为HBV阴性组。取各组对象抗凝全血标本用酚_氯仿法提取DNA进行PCR扩增，并用限制性片段长度多态性检测CYP3A71A和CYP3A71C等位基因。结果：所检测的210份样本CYP3A7均为1A/1A型，未检测到1C等位基因。结论：未检测到CYP3A7高表达1C等位基因，推测CYP3A7在AFB1诱发的HCC发生中所起的作用小。

关键词: 细胞色素P450 3A7, 遗传多态性, 黄曲霉毒素B1, 肝细胞性肝癌

Abstract: BACKGROUND AND AIM: To investigate polymorphisms of CYP3A7 gene of population in Guangxi with heavy aflatoxin B1 (AFB1)contamination, and the relationship between CYP3A7 genotypes and susceptibility of hepatocellular carcinoma (HCC). MATERIALS AND METHODS: Single nucleotide polymorphisms of CYP3A71A and 1C were analyzed in 210 cases and control samples (97 HCC, 38 HBV_positive but with no other disease and 75 HBV_ negative no disease) using the polymerase chain reaction_restriction fragment length polymorphism assays. RESULTS: All of the samples were CYP3A71A/1A genotype ,CYP3A71C allele was not detected in this cohort of Guangxi population. CONCLUSION: CYP3A71C allele was not detected in this cohort,suggesting that CYP3A7 might have a small influence on the development of AFB1_related HCC.

Key words: cytochromes P450 3A7, genetic polymorphism, aflatoxin B1, hepatocellular carcinoma

陆海霞/ 冯震博/冯小兰. 广西黄曲霉毒素B1高污染区210人CYP3A7基因多态性检测[J]. 癌变·畸变·突变, 2008, 20(4): 292-294.

LU Hai_xia, FENG Zhen_bo, FENG Xiao_lan. Polymorphisms of CYP3A7 Gene of 210 Persons in Guangxi with Heavy Aflatoxin B1 Contamination[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(4): 292-294.

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广西黄曲霉毒素B1高污染区210人CYP3A7基因多态性检测

Polymorphisms of CYP3A7 Gene of 210 Persons in Guangxi with Heavy Aflatoxin B1 Contamination

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