NS-398联合奥曲肽对人结肠癌细胞生长影响的研究

doi:10.3969/j.issn.1004-616x.2010.01.006

癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (1): 24-0027.doi: 10.3969/j.issn.1004-616x.2010.01.006

NS-398联合奥曲肽对人结肠癌细胞生长影响的研究

冯振中1;李楠1;陈嘉薇2;葛霞1

1. 蚌埠医学院病理学教研室，蚌埠医学院第一附属医院临床病理科，安徽蚌埠233003； 2. 上海交通大学附属第一人民医院病理科，上海 200080

收稿日期:2009-06-22 修回日期:2009-09-30 出版日期:2010-01-30 发布日期:2010-01-30
通讯作者: 冯振中

Combined inhibitory effects of selective cyclooxygenase-2 inhibitor NS-398 and octreotide on the growth of human colorectal carcinoma

FENG Zhen-zhong1;LI Nan1;CHEN Jia-wei2;GE Xia1

1.Department of Pathology, Bengbu Medical College, Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233003, Anhui; 2.Department of Pathology, The First People's Hospital, Shanghai Jiaotong University, 200080, Shanghai, China

Received:2009-06-22 Revised:2009-09-30 Online:2010-01-30 Published:2010-01-30
Contact: FENG Zhen-zhong

摘要/Abstract

摘要： 目的：探讨联合应用选择性环氧合酶-2(cyclooxyenase-2，COX-2)抑制剂NS-398与奥曲肽对人结肠癌细胞增殖和凋亡的影响。方法：体外培养人结肠腺癌Lovo细胞，分别用 NS-398(100 μmol/L)和奥曲肽(1 μmol/L)单独及联合处理24、48和72 h后，采用MTT法检测细胞的增殖，透射电镜观察细胞凋亡形态学变化，流式细胞仪检测细胞凋亡率和细胞周期改变，RT-PCR检测COX-2基因的表达。结果： NS-398和奥曲肽对Lovo肿瘤细胞的生长具有明显的抑制作用(P<0.05)，且存在时间依赖性，联合应用组抑制效应明显高于单一用药组(P<0.05)。透射电镜观察可见典型的细胞凋亡形态改变。NS-398联合奥曲肽诱导Lovo细胞的凋亡率明显高于单一用药组和对照组。细胞周期分析表明，与对照组比较，各处理组S期细胞比例降低，G0/G1期细胞比例增高(P<0.05)。各处理组均使Lovo细胞COX-2基因mRNA表达下调(P<0.05)。结论： NS-398联合奥曲肽可协同抑制人结肠腺癌Lovo细胞增殖并诱导其凋亡，其机制可能与细胞周期阻滞和下调COX-2基因表达有关。

关键词: 结肠癌, 环氧合酶抑制剂, 奥曲肽

Abstract: OBJECTIVE: To study the effects of selective cyclooxygenase-2 inhibitor NS-398 combined with octreotide on growth and apoptosis of human colon carcinoma cell. METHODS: Lovo cells were treated with NS-398, octreotide or both. The inhibitory effect on the proliferation of Lovo cells was measured by MTT assay. Morphologic changes were examined by electron microscopy, apoptotic percentage and cell cycle of Lovo cells were measured by flow cytometry. The expression of COX-2 mRNA was detected by RT-PCR. RESULTS: NS-398 and octreotide markedly inhibited cells growth in a time-dependent manner, combined treatment could significantly enhance the inhibitory effect than either alone (P<0.05). Apoptotic cells were studied with electron microscope. The apoptotic ratio induced by combined treatment was hihger than other groups. Furthermore, cell cycle analysis showed that S phase cells were decreased and quiescent G0/G1 phase cells accumulated (P<0.05). Compared with control group, the levels of COX-2 mRNA was down-regulated in three experimental groups (P<0.05). CONCLUSION: NS-398 combined with octreotide could synergistically inhibit growth and induce apoptosis of colon carcinoma cell, which may be attributed to cell cycle block and decrease in COX-2 mRNA expression.

Key words: colon carcinoma, cyclooxygenase inhibitor, octreotide

中图分类号:

R735.3

冯振中, 李楠, 陈嘉薇, 葛霞. NS-398联合奥曲肽对人结肠癌细胞生长影响的研究[J]. 癌变·畸变·突变, 2010, 22(1): 24-0027.

FENG Zhen-zhong, LI Nan, CHEN Jia-wei, GE Xia. Combined inhibitory effects of selective cyclooxygenase-2 inhibitor NS-398 and octreotide on the growth of human colorectal carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(1): 24-0027.

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NS-398联合奥曲肽对人结肠癌细胞生长影响的研究

Combined inhibitory effects of selective cyclooxygenase-2 inhibitor NS-398 and octreotide on the growth of human colorectal carcinoma

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