关键词: 聚丁二酸丁二醇酯, 遗传毒性, 染色体畸变, 微核试验

Abstract:

OBJECTIVE: To evaluate the in vitro genotoxicity of PBS [poly(butylene succinate)] in human peripheral blood mononuclear cells. METHODS：The cytotoxicity in PBMCs of PBS including different concentrations (200，100，50，25，12.5，6.25，3.125 mg/mL) was assessed by MTT and the genotoxicity of extracts was evaluated by chromosomal aberration test and micronucleus test. Meanwhile RPMI-1640 and medical biodegradable L-PLA (L-polylactide) were used as control groups. RESULTS：There was little toxicity in the extracts of PBS and no IC50 was valued. There were no cells with chromosomal aberration after co-culture with 200 mg/mL PBS for 48 h under metabolic and non-metabolic activation. The results of micronucleus test compared with the RPMI-1640 and L-PLA groups were not significantly different (P＞0.05). CONCLUSION：PBS showed less genotoxicity in peripheral blood mononuclear cells under this experimental condition. It may be a promising medical biodegradable polymer because of excellent biocompatibility.

Key words: poly(butylene succinate), genotoxicity, chromosomal aberration, micronucleus test