Prohibitin 3'-UTR 基因多态性与粤东乳腺癌发病风险的研究

doi:10.3969/j.issn.1004-616x.2013.04.011

癌变·畸变·突变

Prohibitin 3'-UTR 基因多态性与粤东乳腺癌发病风险的研究

彭琳¹,黄裔腾²，*,罗育豪¹

1. 汕头大学医学院附属肿瘤医院检验科，广东汕头 515041；2. 汕头大学医学院肿瘤研究中心，广东汕头 515041

收稿日期:2013-04-08 修回日期:2013-05-23 出版日期:2013-07-30 发布日期:2013-07-30
通讯作者: 黄裔腾，E-mail：g_ythuang@stu.edu.cn
作者简介: 琳(1980- )，女，广东省汕头市人，硕士，主治医师，研究方向：肿瘤分子诊断。
基金资助:
广东省科技计划资助项目(2009B030801316)，广东省医学科研基金(B2012258)

Prohibitin 3'-untranslated region polymorphism and breast cancer risk in eastern Guangdong of southern China

PENG Lin¹，HUANG Yi-teng²，*，LUO Yu-hao¹

1. Clinical Laboratory, Cancer Hospital of Shantou University Medical College, Shantou 515041; 2. Cancer Research Center, Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2013-04-08 Revised:2013-05-23 Online:2013-07-30 Published:2013-07-30
Contact: HUANG Yi-teng，E-mail：g_ythuang@stu.edu.cn

摘要/Abstract

摘要：

目的：探讨Prohibitin (PHB) 3'-非翻译区 (untranslated region，UTR)基因多态性与粤东汉族女性乳腺癌易感性的关系。方法：采用病例-对照研究，收集231例乳腺癌患者和169例健康对照，提取血液基因组DNA，利用高分辨率熔解曲线 (high resolution melting，HRM)法对PHB基因单核苷酸多态性位点rs1049620、rs9893420、rs4987083进行基因分型，并对检测结果进行非条件Logistic回归分析和χ2检验。结果：PHB基因rs9893420和rs4987083位点在病例组和对照组人群均表现为单一基因型，rs1049620三种基因型G/G、A/G、A/A在病例组和对照组间分布差异均无统计学意义(P>0.05)，在不同组织病理学分型和不同激素受体表达病例中分布差异亦无统计学意义(P>0.05)。结论：未发现PHB基因3'-UTR SNP与粤东汉族女性乳腺癌易感性存在关联。

关键词: 乳腺癌, Prohibitin, 单核苷酸多态性, 高分辨率熔解曲线

Abstract:

OBJECTIVE: To determine the association of Prohibitin (PHB) 3'- untranslated region (UTR) polymorphism with the risk of breast cancer in eastern Guangdong of southern China．METHODS：A case-control study of 231 breast cancer patients and 169 healthy controls were enrolled into this study. Genomic DNA was extracted from whole blood cells. Single nucleotide polymorphisms of rs1049620，rs9893420 and rs4987083 were determined by the high resolution melting (HRM) method．Data were analyzed via Logistic regression analysis and Chi-square test. RESULTS：PHB gene at rs9893420 and rs4987083 sites showed single genotype in breast cancer group and control. There were no significant differences in frequencies of G/G，A/G and A/A at rs1049620 between patients and controls(P>0.05). Meanwhile，no significant correlation could be found between rs1049620 SNP and clinico-pathologic characteristics of breast cancer such as histological classification and expression of hormone receptor. CONCLUSION： Our results do not lend support to the hypothesis that PHB polymorphism contributes to susceptibility of breast cancer.

Key words: breast cancer, Prohibitin, single nucleotide polymophism, high resolution melting

彭琳,黄裔腾,罗育豪. Prohibitin 3'-UTR 基因多态性与粤东乳腺癌发病风险的研究[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2013.04.011.

PENG Lin，HUANG Yi-teng，LUO Yu-hao. Prohibitin 3'-untranslated region polymorphism and breast cancer risk in eastern Guangdong of southern China[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.04.011.

参考文献

[1] Nuell MJ，Stewart DA，Walker L，et al. Prohibitin，an evolutionarily conserved intracellular protein that blocks DNA synthesis in normal fibroblasts and HeLa cells[J]. Mol Cell Biol，1991，11 (3)：1372-1381.

[2] Jupe ER，Badgett AA，Neas BR. Single nucleotide polymorphism in prohibitin 3'untranslated region and breast-cancer susceptibility [J]. Lancet，2001，357 (9268)：1588–1589.

[3] 彭琳，陈建良，方裕森，等. Prohibitin rs6917 C>T基因多态性与潮汕女性乳腺癌易感性的关系研究[J]. 中国医药导报，2012，9 (31)：16-20

[4] DeSantis C，Siegel R，Bandi P，et al. Breast cancer statistics， 2011[J]. CA：Cancer J Clin，2011，61：409–418.

[5] Jupe ER，Liu XT，Kiehlbauch JL，et a1．Prohibitin in breast cancer cell lines：LOSS of antiproliferative activity is linked to 3' untranslated region mutations[J]．Cell Growth Differ，1996， 7 (7)：871-878．

[6] Spurdle AB，Hopper JL，Chen X，et al．Prohibitin 3'untranslated region polymorphism and breast cancer risk in Australian women[J]．Lancet，2002，360 (9337)：925-926．

[7] Campbell IG，Allen J，Eccles DM．Prohibitin 3'untranslated region polymorphism and breast cancer risk[J]．Cancer Epidemiol Biomarkers Prev，2003，12 (Sup 1)：1273-1274．

[8] Karakus N，Kara N，Ulusoy AN．Lack of association between Prohibitin 3'untranslated region C→T polymorphism and breast cancer in a Turkish population[J]．DNA Cell Biol，2008，27 (8)：449-452．

[9] Jakubowska A，Jaworska K，Cybulski C，et al．Do BRCA1 modifiers also affect the risk of breast cancer in non-carriers?[J]．Eur J Cancer，2009，45 (5)：837-842．

[10] Jakubowska A，Gmnwaid J，Grrski B，et al．The 3'untranslated region C> T polymorphism of prohibitin is a breast cancer risk modifier in Polish women carrying a BRCA1 mutation[J]．Breast Cancer Res Treat，2007，104 (1)：67-74．

[11] Zhou TB，Yin SS，Huang JJ，et al. Relationship between the prohibitin 3' untranslated region C > T gene polymorphism and cancer susceptibility ——results of a meta-analysis[J]. Asian Pac J Cancer Prev，2012，13 (7)：3319-3323.

[12] Weizmann Institute of Science. PHB Gene protein-coding GIFtS：65[DB/OL]. (2013-04-08) http://www.genecards.org/ cgi-bin/carddisp.pl?gene=PHB&snp=215#snp.

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Prohibitin 3'-UTR 基因多态性与粤东乳腺癌发病风险的研究

Prohibitin 3'-untranslated region polymorphism and breast cancer risk in eastern Guangdong of southern China

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