甘肃汉族妇女XRCC1和CCNH基因多态性与乳腺癌及乳腺良性肿瘤易感性研究

doi:10.3969/j.issn.1004-616x.2014.04.012

癌变·畸变·突变

甘肃汉族妇女XRCC1和CCNH基因多态性与乳腺癌及乳腺良性肿瘤易感性研究

王兰¹，朱公建¹，闵建平¹，郭红云¹，杨碎胜²，张斌明²，胡清荣¹，杨凯¹，陈学忠²，苏海翔^1，*

甘肃省医学科学研究院转化医学研究中心，甘肃兰州 730050

收稿日期:2013-11-04 修回日期:2184-02-12 出版日期:2014-07-30 发布日期:2014-07-30
通讯作者: 苏海翔，E-mail：haixiang01@yahoo.com
作者简介:王兰(1971- )，女，高级实验师，研究方向：分子生物学。E-mail：wala6535@163.com
基金资助:
甘肃省科技厅支撑项目(1011FKCA089)，甘肃省卫生行业计划项目(GSWST2010-08)

The relevance of XRCC1 and CCNH gene polymorphism to breast cancer and benign breast tumor in Han women of Gansu province

WANG Lan¹，ZHU Gong-jian¹，MIN Jian-ping¹，GUO Hong-yun¹，YANG Sui-sheng²， ZHANG Bin-ming²，HU Qing-rong¹，YANG Kai¹，CHEN Xue-zhong²，SU Hai-xiang^1,*

Center of Translational Medicine Research, Gansu Provincial Academy of Medical Sciences, Lanzhou 730050

Received:2013-11-04 Revised:2184-02-12 Online:2014-07-30 Published:2014-07-30

摘要/Abstract

摘要：

目的：研究甘肃地区汉族妇女XRCC1 rs25487、CCNH rs2234942基因多态性与乳腺癌及乳腺良性肿瘤发病的相关性。方法：选取经病理组织学确诊的乳腺癌、乳腺良性肿瘤各101例，匹配相同数量健康人作对照。使用聚合酶链式反应-限制性片段长度多态分析技术(PCR-RFLP)对XRCC1、CCNH进行基因型分析，通过Logistic回归分析不同基因型和临床病理特征与乳腺癌发病的风险性关系，通过χ2检验比较两种基因位点不同基因型的初潮年龄、发病年龄与乳腺癌和乳腺良性肿瘤的相关性。结果：Logistic回归分析发现XRCC1 rs25487位点GG基因型携带者的妇女罹患乳腺癌的危险性增加(P=0.001， OR=6.39， 95%CI： 2.18～ 18.65)；临床病理免疫组化分析显示，XRCC1基因 rs25487位点携带AA/AG基因型者，在PR+与PR-乳腺癌组织间的分布差异有显著性(P=0.04，OR=0.29)；携带AG/GG基因型者，在Her-2+与Her-2-乳腺癌组织间的分布差异有显著性(P=0.008， OR=0.45)。χ2检验显示，乳腺癌和乳腺良性肿瘤患者XRCC1 rs25487位点GG/AG基因型携带者的初潮年龄差异有显著性(P=0.001、0.043)；乳腺癌和乳腺良性肿瘤患者CCNH rs2234942位点GG基因型携带者的初潮年龄差异有显著性(P=0.049)；乳腺癌和乳腺良性肿瘤患者XRCC1 rs25487和CCNH rs2234942位点GG基因型携带者，发病年龄差异有显著性(P=0.019、0.048)。结论：XRCC1 rs25487 GG基因型将会增加乳腺癌的发病风险，XRCC1 rs25487位点携带AA/AG基因型者，在PR+时乳腺癌的发病风险下降；携带AG/GG基因型者，在Her-2+时可能降低乳腺癌的发病风险。

关键词: XRCC1, CCNH, 基因多态性, 乳腺癌

Abstract:

OBJECTIVE: To study the association between XRCC1 rs25487 and CCNH rs2234942 polymorphism with the susceptibility to breast cancer and benign breast tumor in Han women in Gansu area. METHODS：Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used for the polymorphism of XRCC1，CCNH in 101 cases of breast cancer，101 benign breast tumors and 101 disease-free controls collected in Gansu. Logistic regression analysis was used for comparing the genotypes or clinical pathological characteristics with the risk of breast cancer. Chi-square test was used to compare menarche age and disease onset age with the risk of breast cancer or benign tumor. RESULTS：Logistic regression analysis showed that XRCC1 rs25487，GG genotype increased the risk of breast cancer (P=0.001，OR=6.39，95%CI：2.18－18.65). Regarding the clinicopathological immunohistochemical characteristics，the distribution of AA/AG genotype was significantly different between PR+ and PR- at XRCC1 rs25487(P=0.04，OR=0.29)，while the distribution of AG/GG genotype was significantly different in Her-2+ cases and Her-2- (P=0.008，OR=0.45). Chi-square test found that the menarche age of GG/AG genotype in XRCC1 rs25487 and GG genotype in CCNH rs2234942 was significantly different in breast cancer and benign tumor (P= 0.001， 0.043，0.049). The disease onset age of GG genotype in both XRCC1 rs25487 and CCNH rs2234942 was significantly different in breast cancer and benign tumor(P=0.019，0.048). CONCLUSION： GG genotype in XRCC1 rs25487 increased the risk of breast cancer. AA/AG genotype in XRCC1 rs25487 with PR+ and AG/GG genotype in CCNH rs2234942 with Her-2+ reduced the risk of breast cancer.

Key words: RCC1, CCNH, SNPs, breast cancer

王兰，朱公建，闵建平，郭红云，杨碎胜，张斌明，胡清荣，杨凯，陈学忠，苏海翔. 甘肃汉族妇女XRCC1和CCNH基因多态性与乳腺癌及乳腺良性肿瘤易感性研究[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2014.04.012.

WANG Lan，ZHU Gong-jian，MIN Jian-ping，GUO Hong-yun，YANG Sui-sheng， ZHANG Bin-ming，HU Qing-rong，YANG Kai，CHEN Xue-zhong，SU Hai-xiang. The relevance of XRCC1 and CCNH gene polymorphism to breast cancer and benign breast tumor in Han women of Gansu province[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.04.012.

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甘肃汉族妇女XRCC1和CCNH基因多态性与乳腺癌及乳腺良性肿瘤易感性研究

The relevance of XRCC1 and CCNH gene polymorphism to breast cancer and benign breast tumor in Han women of Gansu province

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