阿特拉津对大鼠多巴胺神经元凋亡相关因子表达的影响

doi:10.3969/j.issn.1004-616x.2015.02.009

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (2): 121-124.doi: 10.3969/j.issn.1004-616x.2015.02.009

阿特拉津对大鼠多巴胺神经元凋亡相关因子表达的影响

李家男, 宋肖垚, 吴沿萍, 李百祥

哈尔滨医科大学公共卫生学院卫生毒理学教研室, 黑龙江哈尔滨 150081

收稿日期:2014-09-01 修回日期:2014-12-02 出版日期:2015-03-31 发布日期:2015-03-31
通讯作者: 李百祥,E-mail:libaix@ems.hrbmu.edu.cn E-mail:libaix@ems.hrbmu.edu.cn
作者简介:李家男,Tel:15124577233,E-mail:364453372@qq.com
基金资助:
国家自然科学基金资助项目(81273109)

Preliminary study of atrazine on the apoptosis of dopaminergic neurons in rats

LI Jia'nan, SONG Xiaoyao, WU Yanping, LI Baixiang

Department of Toxicology, Public Health College of Harbin Medical University, Harbin 150081, Heilongjiang, China

Received:2014-09-01 Revised:2014-12-02 Online:2015-03-31 Published:2015-03-31
About author:10.3969/j.issn.1004-616x.2015.02.009

摘要/Abstract

摘要：

目的:探讨阿特拉津对多巴胺神经元凋亡相关因子表达的影响。方法:将7~8月龄的SD雄性大鼠40只随机分为4组,即对照组和阿特拉津低(10 mg/kg)、中(50 mg/kg)、高(100 mg/kg)剂量组,每组10只,连续灌胃90 d后处死,收集中脑黑质部分,通过透射电镜观察神经元细胞的凋亡情况,分别采用Real-time PCR和Western blot检测凋亡相关基因Bax、Bcl-2、Caspase-3 mRNA和蛋白的表达情况。结果:与对照组相比,随阿特拉津染毒剂量增加,电镜下神经元细胞线粒体出现肿胀、空泡以及线粒体自噬等现象;Bax mRNA及蛋白的表达随剂量增加而增加(P均<0.05);Caspase-3的mRNA在低剂量下表达增高(P均<0.05),但在中、高剂量下表达下降(P均<0.05),其蛋白的表达量随剂量的增加而增加(P均<0.05);但Bcl-2 mRNA及蛋白的表达随剂量增加均呈下降趋势,差异均有统计学意义(P均<0.05)。结论:阿特拉津可通过调节线粒体凋亡相关因子的表达促进多巴胺神经元凋亡。

关键词: 阿特拉津, 多巴胺神经元, 凋亡, Bax, Bcl-2, Caspase-3

Abstract:

OBJECTIVE:To study the effects of atrazine(ATR) on apoptosis of dopaminergic neurons. METHODS:The 7-8 months old SD male rats were randomly divided into four groups, control group and ATR-dose groups (10, 50, 100 mg/kg). The rats were gavaged for 90 days then sacrificed. Midbrain nigra was collected and stored frozen in -80 ℃. We used transmission electron microscope to examine the apoptosis of dopaminergic neurons, Real-time PCR to measure apoptotic genes Bax, Bcl-2 and Caspase-3 mRNA expression levels and Western blot to evaluate apoptotic proteins Bax and Caspase-3 expression levels. RESULTS:Compared with the control group, with increasing dose, dopaminergic neurons developed mitochondrial swelling, vacuoles and mitochondrial autophagy. Compared with the control group, the Bax mRNA and protein levels were significantly increased (P<0.05), whilst the Bcl-2 mRNA and protein levels of each group were significantly lower than control group(P<0.05). The expression of Capase-3 mRNA level in 10 mg/kg group was significantly higher than control group (P<0.05) and in the 50 and 100 mg/kg groups were significantly lower than control group (P<0.05). The expressions of Caspase-3 protein levels were increased with increasing does in the medium and high dose groups(P<0.05). CONCLUSION:Atrazine, through mitochondrial apoptotic signal could cause the death of neurons.

Key words: Atrazine, dopaminergic neurons, apoptosis, Bax, Bcl-2, Caspase-3

中图分类号:

R114

李家男, 宋肖垚, 吴沿萍, 李百祥. 阿特拉津对大鼠多巴胺神经元凋亡相关因子表达的影响[J]. 癌变·畸变·突变, 2015, 27(2): 121-124.

LI Jia'nan, SONG Xiaoyao, WU Yanping, LI Baixiang. Preliminary study of atrazine on the apoptosis of dopaminergic neurons in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(2): 121-124.

参考文献

[1] Lidia A, Rosamafia L, Antonio M, et al. G-protein-coupled receptor 30 and estrogen receptor-d are involved in the proliferative effects induced by atrazine in ovarian cancer cells[J]. Environ Health Perspect, 2008, 116(12):1648-1655.
[2] Foradori CD, Hinds LR, Hanneman WH, et al. Effects of atrazine and its withdrawal on gonadotropin-relrasing hormone neuroen-docrine function in the adult female Wistar rat[J]. Biol Reprod, 2009, 81(6):1099-1105.
[3] 张逸帆, 倪沙, 邓双丽, 等. 阿特拉津对动物生长发育影响的研究进展[J]. 农业生态科学, 2008, 24(11):424-427.
[4] 李术, 付瑶, 李铭, 等. 阿特拉津和毒死蜱对鲤鱼鳃组织细胞色素P450酶系影响[J]. 东北农业大学学报, 2013, 44(6):107-111.
[5] Sean DW. Comber, Abiotic persistence of atrazine and simazine in water[J]. Pesti Sci, 1999, 55(7):696-702.
[6] Bardullas U, Giordano M, Rodríguez A. Chronic atrazine exposure causes disruption of the spontaneous locomotor activity and alters the striatal dopaminergic system of the male Sprague-Dawley rat[J]. Neurotoxicol Teratol, 2011, 33(2):263-272.
[7] Hayes WJ, Laws, ER. Handbook of Pesticide Toxicology[M]. USA:Academic Press, 1991:816-822.
[8] 秦传玉, 赵勇胜, 刘娜, 等. 阿特拉津在土壤中的吸附行为[J]. 环境污染与防治, 2007, 29(3):165-167.
[9] Brown TP, Rumsby PC, Capleton AC, et al. Pesticides and Parkinson's disease-is there a link?[J]. Environ Health Perspect, 2006, 114(2):156-164.
[10] Bonvicini F, Marcello N, Mandrioli J, et al. Exposure topesticides and risk of amyotrophic lateral sclerosis:a population-based case-control study[J]. Annali dell'Istituto Superiore di Sanità, 2010, 46(3):284-287.
[11] Betarbet R, Sherer TB, MacKenzie G, et al. Chronic systemic pesticide exposure reproduces features of Parkinson's disease[J]. Nature Neurosci, 2000, 3(12):1301-1306.
[12] Castello PR, Drechsel DA, Patel M. Mitochondria are a major source of paraquat-induced reactive oxygen species production in the brain[J]. J Biol Chem, 2007, 282:14186-14193.
[13] Chun HS, Gibson GE, DeGiorgio LA, et al. Dopaminergic cell death induced by MPP(+), oxidant and specific neurotoxicants shares the common molecular mechanism[J]. J Neurochem, 2001, 76(4):1010-1021.
[14] Dodd PR. Excited to death:different ways to lose your neurones[J]. Biogerentol, 2002, 3(1/2):51-56.

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阿特拉津对大鼠多巴胺神经元凋亡相关因子表达的影响

Preliminary study of atrazine on the apoptosis of dopaminergic neurons in rats

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