关键词: miR-34a, 宫颈癌, 临床特征, ROC曲线, 维吾尔族

Abstract: OBJECTIVE: To investigate the expression of miR-34a in different types of cervical lesions from Uyghur and Han women in Xinjiang,and to explore its association with clinicopathologic parameters of cervical carcinoma and its clinical value. METHODS: Quantitative real-time PCR was used to detect miR-34a expression in 58 specimens of cervical carcinoma,60 specimens of cervical intraepithelial neoplasia Ⅱ-Ⅲ level (CINⅡ-Ⅲ level)and 32 specimens of healthy control tissue. Data were used to analyze correlations between the expression levels of miR-34a in cervical lesions to different degrees of Uyghur and Han women and related clinicopathologic parameters of cervical carcinoma. Receiver operating characteristic curve (ROC curve) were used to analyze the ability of potential biomarkers to determine cervical carcinoma. RESULTS: The expression of miR-34a in cervical carcinoma was statistically significantly lower than those in CINⅡ-Ⅲ level and healthy control tissue (P<0.05),but no obvious difference was detected between women with Uyghur and Han ethnicities (P>0.05). The expression of miR-34a was associated with the tumor size and FIGO stage and lymph node metastasis (P<0.01). No significant associations were found between miR-34a level and other clinicopathologic parameters of cervical carcinoma,such as age,differentiation,pathological types (P>0.05). miR-34a yielded a receiver-operator characteristic curve area(AUC) of 0.889 (sensitivity 87.5% and specificity 81.0%) to discriminating cervical carcinoma patients from healthy controls and 0.767 (sensitivity 78.3% and specificity 70.7%) to discriminating patients with cervical carcinoma from CINⅡ-Ⅲ level patients and 0.810 (sensitivity 83.7% and specificity 70.7%) to determining cervical carcinoma from all cases. CONCLUSION: Expression of miR-34a was similar for the different ethnic groups. Down-regulation of miR-34a may be involved in the oncogenesis and development of cervical carcinoma. Therefore,miR-34a may be useful as a novel biomarker for early diagnosis and prognosis monitoring.

Key words: miR-34a, cervical carcinoma, clinicopathologic features, receiver operating characteristic curve, Uyghur