肺癌干细胞培养、鉴定及其应用的研究进展

doi:10.3969/j.issn.1004-616x.2016.04.016

摘要/Abstract

摘要： 肺癌的发病率呈逐年上升趋势，由于缺乏有效的早期诊断方法以及治疗手段，其5年生存率较低，是威胁人类健康的一大难题。目前治疗方法主要以手术和放化疗为主。“肿瘤干细胞学说”的提出为肺癌的研究开辟了一条新的途径，它被认为是肿瘤复发的根源，那么其分离和鉴定就显得尤为重要。其中肺癌干细胞的主要分离方法有SP细胞分选法、利用干细胞表面标志分选法、利用免疫磁珠分选法联合无血清悬浮培养法、放化疗诱导筛选法等；肺癌干细胞初步鉴定的主要方法有增殖能力、分化能力、耐放化疗性、侵袭能力、致瘤性鉴定等。肺癌干细胞的分离将为实现早期诊断、靶向治疗、基因疗法及药物干预等打下基础，为治疗肺癌，甚至彻底根除肺癌带来了新的希望。本文主要就肺癌干细胞的分离培养、鉴定及其在肺癌治疗方面的应用等的研究进展作一综述。

关键词: 干细胞, 肺癌, 分离, 鉴定

中图分类号:

Q813.1

夏学阳, 郑鑫林, 丁楠, 张建华, 王成, 李斌. 肺癌干细胞培养、鉴定及其应用的研究进展[J]. 癌变·畸变·突变, 2016, 28(4): 321-324.

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