季节对狐狸精子发生的影响

doi:10.3969/j.issn.1004-616x.2016.05.012

癌变·畸变·突变 ›› 2016, Vol. 28 ›› Issue (5): 393-397,406.doi: 10.3969/j.issn.1004-616x.2016.05.012

季节对狐狸精子发生的影响

程丽芳^1,2, 刘明², 朱茂英², 陈德宇²

1. 安徽大学生命科学学院, 安徽合肥 230601;
2. 阜阳师范学院生物与食品工程学院, 安徽阜阳 236037

收稿日期:2016-01-30 修回日期:2016-05-26 出版日期:2016-09-30 发布日期:2016-09-30
通讯作者: 陈德宇,E-mail:chendeyu7104@aliyun.com E-mail:chendeyu7104@aliyun.com
作者简介:程丽芳,E-mail:1289893057@qq.com
基金资助:
安徽省科技攻关项目（12010302066）

Seasonal changes in spermatogenesis in silver fox

CHENG Lifang^1,2, LIU Ming², ZHU Maoying², CHEN Deyu²

1. College of Life Science, Anhui University, Hefei 230601;
2. Biotechnology and Food Engineering, Fuyang Normal University, Fuyang 236037, Anhui, China

Received:2016-01-30 Revised:2016-05-26 Online:2016-09-30 Published:2016-09-30

摘要/Abstract

摘要： 目的：狐狸是短日照季节性单次发情动物，精子的发生有明显的阶段特异性。本研究是为了探明季节变化对狐狸精子发生的影响。方法：以未发情期（2014年9月28日）、睾丸发育初期（2014年11月29日）和睾丸发育成熟期（2015年1月27日）的银狐各2只为研究对象，称量双侧睾丸的质量和体积，采用酶联免疫吸附实验检测睾丸中睾酮的含量。并利用组织切片技术分析曲细精管中各类生精细胞的组成及比例。结果：9月、11月和次年1月银狐睾丸的质量分别为（0.896±0.002）g、（3.365±0.081）g、（5.241±0.100）g；体积分别为（1.215±0.036）cm³、（5.558±0.794）cm³、（14.112±2.854）cm³；睾酮含量分别为（0.211±0.060）μg/g、（2.040±0.202）μg/g、（3.240±0.432）μg/g。从9月至次年1月银狐睾丸质量、体积以及睾酮含量均逐渐增加，组间差异具有统计学意义（P均<0.05）。切片上单个曲细精管横截面中生精细胞的组成和数量随着时间的推移逐渐增加呈现季节变化，组间差异具有统计学意义（P<0.05）。结论：狐狸的精子发生呈现典型的季节性，在皖北采集公狐精液的最佳时间为1月20日左右。本研究为探究银狐的繁殖特性及提高银狐的人工授精率奠定了基础。

关键词: 银狐, 睾丸, 精子发生, 季节变化

Abstract: OBJECTIVE: Fox is a seasonal single-estrus animal and its spermatogenesis is stage-specific. The objective of our study was to ascertain the impact of seasonal variations on silver fox spermatogenesis. METHODS: Silver fox testicles were taken in September,November and January for measurement of testicular volume,weight and other basic morphological parameters. Testosterone in the testes was measured by enzyme-linked assay. Tissue slice seminiferous tubules from all kinds of raw composition and proportion of sperm cells were analyzed. RESULTS: In September,November,January,the average testicular weights were (0.896±0.002) g,(3.365±0.081) g and (5.241 ±0.100) g;volume were (1.215±0.036) cm³,(5.558±0.794) cm³ and (14.112±2.854) cm³ and testosterone contents were (0.211±0.060) μg/g,(2.040±0.202) μg/g,(3.240±0.432) μg/g,respectively. Testicular volume, weight and testosterone concentrations in the three months were significantly different (P<0.05). The content of testosterone and the weight changes were positively correlated (r=0.991). In the different seasons,the composition and number of the spermatogenic cell in seminiferous tubules changed with the seasons significantly (P<0.05). CONCLUSION: Our results indicate that spermatogenesis in silver fox showed typical seasonal patterns. The information can be used to determine the best time of semen collection and the approach to enhance artificial fertilization rate.

Key words: silver fox, testis, spermatogenesis, seasonal changes

中图分类号:

Q492

程丽芳, 刘明, 朱茂英, 陈德宇. 季节对狐狸精子发生的影响[J]. 癌变·畸变·突变, 2016, 28(5): 393-397,406.

CHENG Lifang, LIU Ming, ZHU Maoying, CHEN Deyu. Seasonal changes in spermatogenesis in silver fox[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(5): 393-397,406.

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季节对狐狸精子发生的影响

Seasonal changes in spermatogenesis in silver fox

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