关键词: 阳性对照物, 敌枯双, 环磷酰胺, 阿司匹林, 畸形率, 畸胎类型

Abstract: OBJECTIVE:To provide positive reference for teratogenicity test on food,health care products and cosmetics,we explored teratogenic effects of three different positive control materials. METHODS:According to the gestation time of SD rats,pregnant rats were randomly divided into control,aspirin (280 mg/kg),Bis-A-TDA (1 mg/kg) and CP (12 mg/kg) groups. Their body weights on days 0,6,9,12,15 and 20 of pregnancy were recorded. On day 6-15 of gestation,the aspirin and Bis-A-TDA groups were administrated corresponding solutions,while CP group were injected ip with CP on day 12. Pregnant rats were inspected by cesarean section and weights of uterus,uterine and fetuses were recorded. The number of corpus luteum,implantation point,and embryonic development were examined. Appearance of fetus was examined,and fetal weight,body length and tail length of each fetus were measured. 1/2 of the live fetus were stained for skeletal examination and the others were fixed for visceral examination. RESULTS:In the aspirin group,body weight and weight increment of the pregnant rats,the weight of the uterus and uterine and fetal were reduced. The rates of live fetus,fetal weight,the length of body and tail were decreased significantly (P < 0.05,P < 0.01). In the Bis-A-TDA group,the rates of live fetus,fetal weight,the length of body and tail were significantly decreased (P < 0.01). In the CP group,weight increments of the pregnant rats,and the weight of the uterus and uterine and fetal,and the rate of live fetus were decreased significantly (P < 0.05,P < 0.01). The teratogenic rate of appearance, skeleton and viscera of three different positive control materials were statistically significant (P < 0.01). There were more teratogenic types and higher teratogenic rates in the Bis-A-TDA and CP groups. CONCLUSION:Under the conditions of our experiment,Bis-A-TDA (1 mg/kg) and CP (12 mg/kg) induced more teratogenic types,and higher teratogenic rate,therefore they were more sensitive positive control materials on teratogenicity test.

Key words: positive control material, aspirin, Bis-A-TDA, CP, teratogenic rate, teratogenic type