食管鳞癌ECA109细胞中Survivin通过ERK信号通路调控c-myc基因表达的机制

doi:10.3969/j.issn.1004-616x.2018.05.003

癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (5): 345-348,353.doi: 10.3969/j.issn.1004-616x.2018.05.003

食管鳞癌ECA109细胞中Survivin通过ERK信号通路调控c-myc基因表达的机制

闫冬¹, 吴怡娴², 董娟娟², 李秀梅², 封敏³

1. 新疆医科大学药学院, 新疆乌鲁木齐 830011;
2. 新疆医科大学基础医学院, 新疆乌鲁木齐 830011;
3. 新疆医科大学附属肿瘤医院, 新疆乌鲁木齐 830000

收稿日期:2018-02-07 修回日期:2018-09-14 出版日期:2018-09-30 发布日期:2018-09-30
通讯作者: 封敏,E-mail:dr.lxm@163.com E-mail:dr.lxm@163.com
作者简介:闫冬,E-mail:252036104@qq.com。
基金资助:
新疆维吾尔自治区自然科学基金（2017D01C392）

Regulation of c-myc expression by Survivin via the ERK signal pathway in esophageal ECA109 cells

YAN Dong¹, WU Yixian², DONG Juanjuan², LI Xiumei², FEN Ming³

1. School of Pharmacy, Xinjiang Medical University, Urumqi 830011;
2. School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830011;
3. Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang, China

Received:2018-02-07 Revised:2018-09-14 Online:2018-09-30 Published:2018-09-30

摘要/Abstract

摘要： 目的：探讨在食管鳞癌ECA109细胞中Survivin对c-myc基因的调控作用。方法：将食管鳞癌ECA109细胞分为Survivin shRNA干扰组、阴性质粒对照组（Control shRNA）和空白细胞株（未加任何处理的食管癌ECA109细胞），将2μg Survivin shRNA质粒、2μg Control shRNA质粒分别转染ECA109细胞，48 h后收集各组细胞，采用RT-PCR法检测各组Survivin、c-myc mRNA的表达，Western blot法检测Survivin、c-myc蛋白及p-ERK蛋白的表达；采用ERK、p38、JNK、JAK/STA3、PI3K/Akt信号传导通路的特异性抑制剂分别阻断ECA109细胞中关键激酶的表达，RT-PCR法检测c-myc mRNA的表达。结果：与阴性质粒对照组和空白细胞组比较，Survivin shRNA组Survivin表达下调，c-myc mRNA及蛋白的表达降低，差异均具有统计学意义（P均 < 0.05）；采用ERK、p38、JNK、JAK/STA3、PI3K/Akt信号通路抑制剂分别作用于食管癌ECA109细胞，PD98059（ERK信号通路阻断剂）组c-myc mRNA及蛋白表达降低（P < 0.05）；Survivin shRNA干扰沉默Survivin基因后ERK磷酸化蛋白表达降低（P < 0.05）。结论：Survivin对c-myc具有正向调控作用，可能通过ERK信号传导通路调控c-myc的表达。

关键词: 短发夹RNA, Survivin, c-myc, 胞外信号调节激酶

Abstract: OBJECTIVE:To study regulatory effects of Survivin on c-myc in esophageal ECA109 cells. METHODS:ECA109 esophageal squamous carcinoma cells were divided into Survivin shRNA interference,control shRNA and control groups (without any treatment). ECA109 cells were transfected with 2μg Survivin shRNA and 2μg control shRNA plasmids,respectively. After 48 h,expressions of Survivin,c-myc mRNA were detected by the RT-PCT method,proteins of Survivin,c-myc and p-ERK were detected by Western blot. ERK,p38,JNK,JAK/STAT3, PI3K/Akt signal pathway inhibitors were used to detect mRNA expression of c-myc by half quantitative RT-PCR and Western blot. RESULTS:Compared with the negative plasmid control group and the blank cell group,the expression of c-myc mRNA and protein was decreased after the interference of the Survivin shRNA group (P=0.022,P=0.000). c-myc mRNA and protein expressions were reduced in the ERK signal channel blockers group (P < 0.05). The expression of ERK phosphorylation protein was reduced after Survivin silence in the Survivin shRNA interference group. CONCLUSION:Survivin was effective in regulation of ERK activation state in upstream signal transduction. The role of Survivin in regulation of c-myc expression was related to the ERK signaling pathway.

Key words: shRNA, Survivin, c-myc, ERK

中图分类号:

R73-3

闫冬, 吴怡娴, 董娟娟, 李秀梅, 封敏. 食管鳞癌ECA109细胞中Survivin通过ERK信号通路调控c-myc基因表达的机制[J]. 癌变·畸变·突变, 2018, 30(5): 345-348,353.

YAN Dong, WU Yixian, DONG Juanjuan, LI Xiumei, FEN Ming. Regulation of c-myc expression by Survivin via the ERK signal pathway in esophageal ECA109 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 345-348,353.

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食管鳞癌ECA109细胞中Survivin通过ERK信号通路调控c-myc基因表达的机制

Regulation of c-myc expression by Survivin via the ERK signal pathway in esophageal ECA109 cells

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