百草枯对大鼠血脑屏障及认知记忆功能的影响

doi:10.3969/j.issn.1004-616x.2019.00.009

癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (2): 142-147,171.doi: 10.3969/j.issn.1004-616x.2019.00.009

百草枯对大鼠血脑屏障及认知记忆功能的影响

马慧, 李紫乔, 赵丽, 孙艳

哈尔滨医科大学公共卫生学院劳动卫生与环境卫生学教研室, 黑龙江哈尔滨 150081

收稿日期:2018-05-28 修回日期:2018-12-06 出版日期:2019-03-30 发布日期:2019-03-30
通讯作者: 孙艳,E-mail:13946167049@163.com E-mail:13946167049@163.com
作者简介:马慧,E-mail:13163676217@163.com
基金资助:
国家自然科学基金资助项目（81402711）

Effects of paraquat on blood-brain barrier and cognitive memory function in rats

MA Hui, LI Ziqiao, ZHAO Li, SUN Yan

Department of Labor Health and Environmental Hygiene, College of Public Health, Harbin Medical University, Harbin 150081, Heilongjiang, China

Received:2018-05-28 Revised:2018-12-06 Online:2019-03-30 Published:2019-03-30

摘要/Abstract

摘要： 目的：探讨百草枯（PQ）对大鼠血脑屏障及认知、学习、记忆相关功能的影响。方法：将SPF级SD雄性大鼠45只随机分为3组即对照组（生理盐水）、PQ低剂量（1 mg/kg）和PQ高剂量（10 mg/kg）染毒组，每组15只，腹腔注射6周，每周2次。染毒结束后，每组随机抽取5只大鼠进行伊文思蓝脑渗透实验，检测血脑屏障的通透性。各组其余10只大鼠进行行为学实验，包括Morris水迷宫实验、跳台实验、穿梭箱实验，测试其认知、学习和记忆能力。结果：与对照组比较，2个PQ染毒组大鼠脑组织伊文思蓝含量均增加，差异具有统计学意义（P < 0.05）；Morris水迷宫结果显示，在训练阶段，1和10 mg/kg PQ组大鼠运动轨迹较对照组复杂，随着训练天数增加，各组大鼠的逃避潜伏期均逐渐缩短，10 mg/kg PQ组的缩短趋势低于对照组，差异具有统计学意义（P < 0.05），测试阶段，与对照组相比，1和10 mg/kg PQ组大鼠穿越平台次数明显减少，差异具有统计学意义（P < 0.05）；跳台结果显示，1和10 mg/kg PQ组的记忆潜伏期明显低于对照组，错误次数明显高于对照组，且10 mg/kg PQ组大鼠错误次数高于1 mg/kg PQ组，差异具有统计学意义（P < 0.05）；穿梭箱实验结果表明，2个剂量PQ组大鼠条件反应次数均低于对照组，且错误次数均高于对照组，差异具有统计学意义（P < 0.05）。结论：1和10 mg/kg百草枯均损伤大鼠血脑屏障，增加其通透性，进而降低认知、学习以及记忆功能。

关键词: 百草枯, 血脑屏障, 认知记忆功能, 大鼠

Abstract: OBJECTIVE:To explore the effects of paraquat (PQ) on blood-brain barrier and cognitive,learning and memory-related functions in rats.METHODS:SPF grade SD male rats were randomly divided into three groups:control (normal saline),low dose PQ exposure (1 mg/kg) and high dose exposure (10 mg/kg) groups.There were 15 rats per group and PQ was injected intraperitoneally for 6 weeks at 2 times a week.After the exposure,5 rats in each group were randomly selected for the Evans blue brain penetration test to detect the permeability of the blood-brain barrier.The remaining 10 rats in each group were subjected to behavioral experiments for their cognitive,learning,and memory abilities,including Morris water maze test,platform test,and shuttle box test.RESULTS:Compared with the control group,the content of Evans blue increased in the brain tissue of each dose group,the difference was statistically significant (P < 0.05).Morris water maze results show that the trajectories of rats in the 1 and 10 mg/kg PQ groups were more complex than those in the control group.With the increase of training days,the escape latency of each group was gradually shortened.The shortening trend of the 10 mg/kg PQ group was significantly lower than that of the control and the low dose groups (P < 0.05).During the test period,the number of crossings in the 1 and 10 mg/kg PQ group was significantly reduced compared with the control group (P < 0.05).The results of the platform test show that the memory latency of the 1 and 10 mg/kg PQ group was significantly lower than that of the control group,and the number of errors was significantly higher than that of the control group,and the number of errors in the 10 mg/kg PQ group was significantly higher than that in the 1 mg/kg PQ group (P < 0.05).The results of the shuttle box experiment show that the number of conditional responses in the two dose groups was significantly lower than that in the control group,and the number of errors was significantly higher than that in the control group (P < 0.05).CONCLUSION:Both 1 and 10 mg/kg doses of PQ damaged the blood-brain barrier and increased its permeability which in turn reduced cognitive,learning and memory functions.

Key words: paraquat, blood-brain barrier, cognitive memory function, rats

中图分类号:

S884.9

马慧, 李紫乔, 赵丽, 孙艳. 百草枯对大鼠血脑屏障及认知记忆功能的影响[J]. 癌变·畸变·突变, 2019, 31(2): 142-147,171.

MA Hui, LI Ziqiao, ZHAO Li, SUN Yan. Effects of paraquat on blood-brain barrier and cognitive memory function in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(2): 142-147,171.

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百草枯对大鼠血脑屏障及认知记忆功能的影响

Effects of paraquat on blood-brain barrier and cognitive memory function in rats

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