X射线测定蛋白质结构的技术进展与研究现状

doi:10.3969/j.issn.1004-616x.2019.01.016

癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (1): 82-85.doi: 10.3969/j.issn.1004-616x.2019.01.016

X射线测定蛋白质结构的技术进展与研究现状

熊强, 丁立新, 姜晓燕, 丁库克

中国疾病预防控制中心辐射防护与核安全医学中心放射生态研究室, 北京 100088

收稿日期:2018-02-02 修回日期:2018-10-16 出版日期:2019-01-31 发布日期:2019-01-31
通讯作者: 丁库克,E-mail:shouding@ccmu.edu.cn E-mail:shouding@ccmu.edu.cn
作者简介:熊强,E-mail:304639809@qq.com。
基金资助:
国家自然科学基金资助项目（31640022，31770907）；北京市自然科学基金资助项目（7172146）

Received:2018-02-02 Revised:2018-10-16 Online:2019-01-31 Published:2019-01-31

摘要/Abstract

摘要： X射线晶体衍射技术是一种非常重要的测定蛋白质晶体结构的方法。近几十年，虽然各种新的实验方法和通过计算机预测蛋白质结构域的方法层出不穷，但是测定蛋白质结构主要还是通过X射线晶体衍射技术。随着同步辐射装置和X射线自由电子激光的发展，X射线在测定蛋白质结构中的作用越来越重要。本文就X射线测定蛋白质晶体空间结构的技术进展进行综述，介绍了其原理和发展，简述了同步辐射和X射线自由电子激光的研究现状，为理解用X射线测定蛋白质结构的机制，从原子水平上了解生命物质奠定基础，并提出今后X射线光源的发展趋势。

关键词: 蛋白质晶体结构, X射线晶体衍射技术, 同步辐射, X射线自由电子激光

中图分类号:

O743+.52

熊强, 丁立新, 姜晓燕, 丁库克. X射线测定蛋白质结构的技术进展与研究现状[J]. 癌变·畸变·突变, 2019, 31(1): 82-85.

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X射线测定蛋白质结构的技术进展与研究现状

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