3种市售蚊香液对植物根尖细胞的遗传损伤效应

doi:10.3969/j.issn.1004-616x.2019.05.012

摘要/Abstract

摘要： 目的：电热蚊香液使用广泛，本研究旨在探讨其对植物的遗传损伤效应。方法：以蚕豆、大蒜和洋葱为实验材料，采用植物根尖细胞微核试验，测定在不同浓度（25%、50%和75%）的3种品牌（BBN、JJ和YB）蚊香液处理下，植物根尖细胞的微核率。结果：随着3种蚊香液浓度的增加，植物根尖细胞微核率也随之增加。75%的BBN、JJ和YB蚊香液分别作用于蚕豆根尖细胞时，细胞微核率均显著高于对照组（P < 0.05）；75%的JJ和YB蚊香液分别作用于大蒜根尖细胞时，微核率较对照组差异显著（P < 0.05）；50%和75%的3种蚊香液作用于洋葱根尖细胞时，微核率高于对照组（P < 0.05）；75%的JJ和YB蚊香液处理组洋葱根尖的细胞微核率明显高于蚕豆（P < 0.05）；3种品牌蚊香液对植物根尖细胞遗传损伤效应的差异无统计学意义（P > 0.05）。结论：在本实验条件下，蚊香液达到一定浓度时均对蚕豆、大蒜和洋葱根尖细胞造成遗传损伤，高浓度蚊香液对洋葱的遗传损伤显著高于蚕豆，不同品牌蚊香液对植物产生的损伤程度无明显差异。

关键词: 蚊香液, 蚕豆, 大蒜, 洋葱, 根尖细胞, 微核率

Abstract: OBJECTIVE:Electric mosquito repellents are widely used therefore their toxicologic effects should be better understood. The purpose of this study was to investigate genoxicity of the repellents in plant cells. METHODS:Plant cells were exposed to various concentrations (25%,50% and 75%) of 3 brands of electric mosquito repellents (BBN,JJ and YB electric mosquito liquid). The micronucleus test was used to monitor genotoxic effects. RESULTS:When the Vicia faba root tip cells were exposed to the three brands of electric mosquito repellents at the 75% concentration,the micronucleus rates were significantly higher than that in the untreated control group (P < 0.05). Under the similar experimental conditions using the garlic root tip cells,only the JJ and YB brands showed significant induction of micronuclei (P < 0.05). Under the similar conditions using the onion root tip cells,both the 50% and 75% concentrations induced significantly higher micronuclei frequencies (P < 0.05). The micronucleus rates in the treated onion root tip cells at the 75% concentration were significantly higher than those of Vicia faba (P < 0.05). The genotoxic effects from the three kinds of electric mosquito repellents showed no significance differences (P > 0.05). CONCLUSION:Three brands of electric mosquito repellents caused genotoxicity to root tip cells of Vicia faba,garlic and onion,and the damage to onion cells was significantly higher than that to Vicia faba. There was no statistical difference in the damage caused by three kinds of electric mosquito repellents.

Key words: electric mosquito liquid, Vicia faba, garlic, onion, root tip cells, micronucleus rate

中图分类号:

Q355

李莹, 陈霞明, 李函融, 罗特. 3种市售蚊香液对植物根尖细胞的遗传损伤效应[J]. 癌变·畸变·突变, 2019, 31(5): 401-405,411.

LI Ying, CHEN Xiaming, LI Hanrong, LUO Te. Genotoxicity of three kinds of electric mosquito repellents on plant root tip cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 401-405,411.

参考文献

[1] 颇俊华. 中国蚊香百年进展和人类健康[J]. 环境保护, 2014, 2:9-10.
[2] 徐金英, 张媛. 环保电热液体蚊香的研究[J]. 日用化学品科学, 2012, 35(10):33-35.
[3] 韦金. 挥发调整剂在环保型电热液体蚊香的应用研究[J]. 环境保护, 2014, 1:7-8.
[4] 杜洁. 拟除虫菊酯对斑马鱼内分泌平衡及氧化代谢的扰动效应[D]. 杭州:浙江工业大学, 2016.
[5] Environmental Protection Agency(EPA). Endocrine Disruptor Screening and Testing Advisory Committee[M]. United States:EPA, 1998.
[6] 杜洁, 张怡, 洪盼盼, 等. 拟除虫菊酯对斑马鱼氧化代谢相关基因表达影响[J]. 浙江工业大学学报, 2016, 44(3):334-339.
[7] GODIN S J, CROW J A, SCOLLON E J, et al. Identification of rat and human cytochrome P450 isoforms and a rat serum esterase that metabolize the parathyroid insecticides deltamethrin and esfenvalerate[J]. Drug Metab Dispos, 2007, 35(9):1664-1671.
[8] 王伟, 柴菁, 孙翰昌, 等. 氟氯氰菊酯对草鱼的急性毒性及转氨酶活性的影响[J]. 农业环境科学学报, 2017, 36(12):2388-2393.
[9] 郑盛华, 杨妙峰, 郑惠东, 等. 联苯菊酯和醚菊酯对真鲷的急性毒性和安全性评价[J]. 安徽农业科学, 2017, 45(29):86-88.
[10] 周冬仁, 盛鹏程, 孙博弈, 等. 氰戊菊酯与溴氰菊酯对凡纳对虾的急性毒性[J]. 贵州农业科学, 2018, 46(7):103-105.
[11] 杨宗英, 张一柳, 胡鲲, 等. 溴氰菊酯对中华绒螯蟹肝胰腺氧化胁迫效应和组织结构的影响[J]. 浙江农业学报, 2017, 29(8):1261-1270.
[12] MURANLI F D G. Genotoxic and cytotoxic effects of a pyrethroid insecticide lambda-cyhalothrin on human peripheral blood lymphocytes investigated by chromosome aberration and flow cytometry assays[J]. Fresenius Environ Bull, 2009, 18(9A):1758-1763.
[13] 赵春红, 张力, 姜淑卿, 等. 4种常见残留农药联合作用对小鼠遗传毒性研究[J]. 职业与健康, 2018, 34(20):2774-2781.
[14] 李本长, 谢兴华, 朱若凯, 等. 氟丙菊酯原药对大鼠亚慢性毒性作用的研究[J]. 江西医药, 2018, 53(6):546-560.
[15] 刘伟. 毒死蜱、马拉硫磷和氰戊菊酯对蚯蚓、蚕豆毒性效应的研究[D]. 泰安:山东农业大学, 2006.
[16] 唐历波, 刘燕. 三种农药对大蒜根尖细胞姐妹染色单体互换频率及微核率的影响[J]. 环境与健康杂志, 2009, 26(4):308-310.
[17] 龚得春. 梁滩河流域拟除虫菊酯农药多介质残留和环境行为研究[D]. 重庆:重庆大学, 2013.
[18] 赵鑫磊. 日化用品对蚕豆根尖的微核效应研究[J]. 生物化学, 2016, 2(1):52-54.
[19] 邓冰, 穆龙, 周银行. 采用蚕豆根尖细胞微核技术检测核设施周围水域的遗传毒性[J]. 癌变·畸变·突变, 2016, 28(2):141-148.
[20] 黎杰强, 黄海琳, 李丹霞, 等. 3种常用农药对蚕豆根尖细胞的遗传毒性[J]. 华南师范大学学报(自然科学版), 2008, 1:112-117.
[21] 赵祥, 王金花, 朱鲁生, 等. 抗生素和铜联合作用对蚕豆根尖细胞微核率的影响[J]. 环境科学研究, 2015, 28(7):1085-1090.
[22] 杨玉英, 梁坚, 黄超培, 等. 溴氰菊酯对紫露草微核率的影响[J]. 广西医学, 1993, 15(3):241-242.
[23] 卜宁, 马莲菊, 王升厚. 农药氰戊菊酯和三氟氯氰菊酯对蚕豆根尖细胞的遗传损伤[J]. 生态毒理学报, 2008, 3(1):93-97.
[24] 王兰君, 王金花, 朱鲁生, 等. 4种典型PPCPs对蚕豆和大蒜根尖细胞微核率的影响[J]. 环境科学, 2016, 37(4):1568-1575.
[25] 吴笛, 张国栋, 封少龙, 等. 两种新型杀虫剂对大蒜及蚕豆根尖细胞微核率影响的研究[J]. 农业环境保护, 2002, 21(1):72-73.
[26] 侯家龙, 张庆. 应用大蒜根尖微核技术检测水质污染的初步研究[J]. 生态学杂志, 1988, 7(3):50-52.
[27] 李秋江, 赵云霞, 谢雅舒, 等. 不同浓度重铬酸钾溶液胁迫对洋葱根尖生理生化特性的影响[J]. 湖北农业科学, 2017, 56(8):1489-1493.
[28] 李庆凤, 高菊芳, 谷银芳, 等. 氯氟醚菊酯及其他3种杀虫剂对淡色库蚊和家蝇毒力及室内效果评价[J]. 中国媒介生物学及控制杂志, 2015, 26(3):254-257.
[29] XUE R D, QUALLS W A, PHILLIPS J D, et al. Insectividal activity of five commercial mosquito coils against Anopheles albimanus, Adees albopictus, and Cules quinquefasciatus[J]. J Am Mosq Control Assoc, 2012, 28(2):131-133.
[30] 何建国, 黄琼, 林海, 等. 致倦库蚊对常用卫生杀虫剂抗药性的研究[J]. 中华卫生杀虫药械, 2011, 17(5):364-365.
[31] 谷晓颖, 冯建秀, 仲伟天, 等. 幼鼠重复吸入氯氟醚菊酯蚊香28 d亚急性毒性研究[J]. 预防医学情报杂志, 2013, 29(6):439-442.