MiR-429及Bmi-1 mRNA在弥漫性大B细胞淋巴瘤中的表达及其临床意义

doi:10.3969/j.issn.1004-616x.2020.03.004

癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (3): 177-181.doi: 10.3969/j.issn.1004-616x.2020.03.004

MiR-429及Bmi-1 mRNA在弥漫性大B细胞淋巴瘤中的表达及其临床意义

栾亦然¹, 刘丽源¹, 皎龙¹, 汪月玲¹, 白函瑜², 邹乃祎², 马鸣¹

1. 河北医科大学第四医院检验科, 河北石家庄 050011;
2. 河北医科大学第四医院科研中心, 河北石家庄 050011

收稿日期:2020-03-12 修回日期:2020-04-28 出版日期:2020-05-31 发布日期:2020-06-03
通讯作者: 马鸣,E-mail:maming19830419@163.com E-mail:maming19830419@163.com
作者简介:栾亦然,E-mail:abbycharlotte@foxmail.com。
基金资助:
国家自然科学基金（81703073）；河北省自然科学基金（H2018206115）；河北医科大学大学生创新性实验计划项目

Clinical significance of miR-429 and Bmi-1 mRNA expression in diffuse large B-cell lymphoma

LUAN Yiran¹, LIU Liyuan¹, JIAO Long¹, WANG Yueling¹, BAI Hanyu², ZOU Naiyi², MA Ming¹

1. Department of Laboratory, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011;
2. Research Centre, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China

Received:2020-03-12 Revised:2020-04-28 Online:2020-05-31 Published:2020-06-03

摘要/Abstract

摘要： 目的：研究miR-429和Bmi-1 mRNA在弥漫性大B细胞淋巴瘤（DLBCL）组织中的表达及其临床意义。方法：选取自2014年1月—2015年12月河北医科大学第四医院的45例DLBCL患者瘤组织和28例正常对照人群淋巴结组织，用实时荧光定量反转录PCR（RT-qPCR）技术检测组织中miR-429及Bmi-1 mRNA的表达水平，并分析二者之间的关系及其与患者临床病理指标之间的关系。结果：DLBCL组织中miR-429表达水平较正常淋巴结组织明显降低，而Bmi-1 mRNA的表达水平明显升高（P < 0.01）。miR-429低表达组和Bmi-1 mRNA高表达组患者临床分期、骨髓浸润程度、Ki-67阳性率和IPI积分均分别高于miR-429高表达组和Bmi-1 mRNA低表达组（P < 0.05）。结论：DLBCL组织中miR-429及Bmi-1 mRNA的表达水平与患者病情进展和预后密切相关。组织中低表达miR-429及高表达Bmi-1 mRNA可能是患者临床预后较差的预测指标。

关键词: miR-429, 弥漫性大B细胞淋巴瘤, 临床意义, Bmi-1

Abstract: OBJECTIVE: To study the clinical significance of miR-429 and Bmi-1 mRNA in Diffuse large B-cell lymphoma (DLBCL) tissues. METHODS: Samples of tumor tissues were collected from 45 cases of the DLBCL patients from January 2014 to December 2015,which were compared with 28 normal lymph node tissues. Expressions of miR-429 and Bmi-1 mRNA in tumor tissues and normal lymph node tissues were detected by Reverse transcription quantitative polymerase chain reaction (RT-qPCR). Their relationships with the clinical features of the patients were analysed. RESULTS: The tumor tissues showed significantly decreased expression of miR-429 and increased expression of Bmi-1 mRNA,compared with the normal lymph node tissues. DLBCL patients with low expression of miR-429 and high expression of Bmi-1 tend to have later clinical stages,more prone to bone marrow metastasis,higher Ki-67 positive rate and higher IPI score than patients with high expression of miR-429 and low expression of Bmi-1 mRNA (P < 0.05). CONCLUSION: The results show that the expression levels of miR-429 and Bmi-1 in DLBCL tissues were closely related to the progression and prognosis of the lymphomas. Lower miR-429 expression and higher Bmi-1 mRNA expression in tumor tissues may be the predictors of poorer prognosis.

Key words: miR-429, DLBCL, clinical significance, Bmi-1

中图分类号:

R730.2

栾亦然, 刘丽源, 皎龙, 汪月玲, 白函瑜, 邹乃祎, 马鸣. MiR-429及Bmi-1 mRNA在弥漫性大B细胞淋巴瘤中的表达及其临床意义[J]. 癌变·畸变·突变, 2020, 32(3): 177-181.

LUAN Yiran, LIU Liyuan, JIAO Long, WANG Yueling, BAI Hanyu, ZOU Naiyi, MA Ming. Clinical significance of miR-429 and Bmi-1 mRNA expression in diffuse large B-cell lymphoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 177-181.

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MiR-429及Bmi-1 mRNA在弥漫性大B细胞淋巴瘤中的表达及其临床意义

Clinical significance of miR-429 and Bmi-1 mRNA expression in diffuse large B-cell lymphoma

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