电子线照射对小鼠胰岛素生长因子相关蛋白表达的影响

doi:10.3969/j.issn.1004-616x.2020.06.006

癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (6): 444-447,451.doi: 10.3969/j.issn.1004-616x.2020.06.006

电子线照射对小鼠胰岛素生长因子相关蛋白表达的影响

袁慧, 刘梦雅, 许文黎, 胡波, 张亚娟, 尹晶晶, 岳娟, 李建国

中国辐射防护研究院药物安全性评价中心, 山西太原 030006

收稿日期:2019-08-22 修回日期:2019-11-02 出版日期:2020-12-01 发布日期:2020-12-04
通讯作者: 李建国,E-mail:ljg2547@163.com E-mail:ljg2547@163.com
作者简介:袁慧,E-mail:281562563@qq.com。
基金资助:
山西省实验动物专项基金[2014（k17）]

Effect of electron beam irradiation on expression of insulin growth factor-related protein in mice

YUAN Hui, LIU Mengya, XU Wenli, HU Bo, ZHANG Yajuan, YIN Jingjing, YUE Juan, LI Jianguo

Drug Safety Evaluation Center of China Institute for Radiation Protection, Taiyuan 030006, Shanxi, China

Received:2019-08-22 Revised:2019-11-02 Online:2020-12-01 Published:2020-12-04

摘要/Abstract

摘要： 目的：探讨电子线对胰岛素生长因子-1家族蛋白表达的影响，为研究辐射对肝脏的早期损伤提供依据。方法：采用电子线照射小鼠，照射剂量为1、2、4 Gy，用Western blot法检测照射后12、48和72 h小鼠肝脏中胰岛素生长因子1受体（IGF-1R）、胰岛素生长因子结合蛋白1（IGFBP1）、胰岛素生长因子结合蛋白4（IGFBP4）和胰岛素生长因子1（IGF-1）蛋白表达水平的改变。结果：照射剂量为1 Gy时，与对照组相比，IGF-1蛋白在照射后48 h时表达增加，IGFBP1、IGFBP4和IGF1R蛋白的表达在照射后12、48和72 h均表达减少。照射剂量为2 Gy时，IGF-1和IGFBP4蛋白在照射后48 h时表达增加，其余时间点表达减少。IGFBP1蛋白在照射后12 h时表达增加，48和72 h时表达减少。IGF1R蛋白在照射后3个时间点均呈现为表达减少。照射剂量4 Gy时，肝脏中IGF-1、IGFBP1和IGFBP4蛋白均表达减少，IGF1R蛋白在照射后12 h时表达增加，48、72 h时均表达减少（均为P < 0.05）。结论：电子线照射后小鼠肝脏中胰岛素生长因子-1家族蛋白多以下调表达为主，与前期基因表达的结果相一致，有可能作为反映放射性工作人员早期肝脏损伤的生物标志物之一。

关键词: 电子线, 胰岛素样生长因子, 近交系BALB/c小鼠, 蛋白免疫印迹

Abstract: OBJECTIVE: To investigate the influence of electronic beams on protein expression of the insulin growth factor system and to provide evidence for radiation-induced early damage in livers of mice. METHODS: Mice were irradiated with electron beam at of 0,1,2 and 4 Gy. At 12,48 and 72 h after irradiation,their livers were removed and used for analyses. Western blot was used to detect changes of protein expressions in insulin growth factor 1 receptor (IGF-1R),insulin growth factor binding protein 1 (IGFBP1),insulin growth factor binding protein 4 (IGFBP4),and insulin growth factor 1 (IGF-1). RESULTS: When the irradiation dose was 1 Gy,IGF-1 protein was highly expressed at 48 h after irradiation,and that for IGFBP1,IGFBP4 and IGF1R protein were at 12,48 and 72 h. When the irradiation dose was 2 Gy,IGF-1 and IGFBP4 proteins were up regulated at 48 h after irradiation,and they were down regulated for the rest of the time. IGFBP1 protein was up regulated at 12 h after irradiation,and it was down regulated at 48 h and 72 h. IGF1R protein was down regulated at three time points after irradiation. When the irradiation dose was 4 Gy,the expressions of IGF-1,IGFBP1,and IGFBP4 proteins was down regulated. IGF1R protein was up regulated at 12 h after irradiation,and it was down regulated at 48 and 72 h. CONCLUSION: Our results show that electron beam irradiation caused down-regulation on protein expression of the insulin growth factor-1 system in the liver of mice. Our results are consistent with those performed using PCR. Based on the study,expression of the insulin growth factor-1 system may be useful as a biomarker for radiation-induced liver injury.

Key words: electron beam, insulin-like growth factors, inbred BALB/c mice, Western blot

中图分类号:

R818.03

袁慧, 刘梦雅, 许文黎, 胡波, 张亚娟, 尹晶晶, 岳娟, 李建国. 电子线照射对小鼠胰岛素生长因子相关蛋白表达的影响[J]. 癌变·畸变·突变, 2020, 32(6): 444-447,451.

YUAN Hui, LIU Mengya, XU Wenli, HU Bo, ZHANG Yajuan, YIN Jingjing, YUE Juan, LI Jianguo. Effect of electron beam irradiation on expression of insulin growth factor-related protein in mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 444-447,451.

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电子线照射对小鼠胰岛素生长因子相关蛋白表达的影响

Effect of electron beam irradiation on expression of insulin growth factor-related protein in mice

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