甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化相关m6A修饰异常mRNA的分析

doi:10.3969/j.issn.1004-616x.2021.06.001

癌变·畸变·突变 ›› 2021, Vol. 33 ›› Issue (6): 405-409.doi: 10.3969/j.issn.1004-616x.2021.06.001

• 论著 • 下一篇

甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化相关m⁶A修饰异常mRNA的分析

王苗^1,2, 王全凯^1,2, 李昕苇^1,2, 马顺鹏^1,2, 乌瀚宝栎尔¹, 许建宁^1,2

1. 中国疾病预防控制中心职业卫生与中毒控制所, 北京 100050;
2. 中国疾病预防控制中心化学污染与健康安全重点实验室, 北京 100050

收稿日期:2021-08-26 修回日期:2021-11-03 出版日期:2021-11-30 发布日期:2021-12-04
通讯作者: 许建宁,E-mail:jnx999@263.net E-mail:jnx999@263.net
作者简介:王苗,E-mail:2091419301@qq.com。
基金资助:
国家自然科学基金（81673221）

m⁶A methylation of mRNAs in glycidyl methacrylate-induced malignant transformation of 16HBE cells

WANG Miao^1,2, WANG Quankai^1,2, LI Xinwei^1,2, MA Shunpeng^1,2, WUHAN Baolier¹, XU Jianning^1,2

1. National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050;
2. Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China

Received:2021-08-26 Revised:2021-11-03 Online:2021-11-30 Published:2021-12-04

摘要/Abstract

摘要： 目的： 检测甲基丙烯酸环氧丙酯（GMA）诱导16HBE细胞恶性转化相关mRNA的m⁶A甲基化修饰水平，筛选出m⁶A修饰的mRNA并进行功能注释。方法： GMA（8 μg/mL）重复染毒16HBE细胞后，收集GMA染毒组和DMSO对照组的第30代细胞，采用高通量人类表观转录组芯片检测16HBE细胞恶性转化相关mRNA的m⁶A修饰水平，应用Visual Studio Code软件进行m⁶A修饰mRNA的筛选，并利用Omicshare工具对这些mRNA进行GO富集分析和KEGG通路分析。结果： 与对照组细胞比较，GMA诱导的恶性转化16HBE细胞中m⁶A修饰水平异常的mRNA共有454个，其中高甲基化水平mRNA 334个，低甲基化水平mRNA 120个；表达水平异常的mRNA共有434个，其中高表达mRNA 236个，低表达mRNA 198个。m⁶A修饰的mRNA有45个，GO富集分析结果显示上述mRNA主要富集于SNAP受体活性、SNARE结合、SNARE复合体等生物学过程，KEGG通路分析主要富集于囊泡运输中的SNARE相互作用、非同源末端连接、苯丙氨酸代谢等通路。结论： m⁶A修饰mRNA可能在GMA诱导16HBE恶性转化过程发挥重要作用。

关键词: N⁶-甲基腺嘌呤, 甲基丙烯酸环氧丙酯, 高通量基因芯片, 恶性转化细胞

Abstract: OBJECTIVE: To investigate m⁶A methylation levels of mRNAs which are related to glycidyl methacrylate (GMA)-induced malignant transformation of 16HBE cells and their functional roles. METHODS: After 16HBE cells were repeatedly exposed to GMA(8 μg/mL),the 30th generation cells of GMA group and DMSO control group were collected. m⁶A methylation levels of mRNAs which were related to 16HBE malignant transformation were detected by high-throughput human apparent transcriptome chip. The differentially m⁶A-methylated mRNAs and differentially expressed mRNAs were screened by Visual Studio Code. The Omicshare tools were used for GO enrichment and KEGG pathway analyses of these mRNAs. RESULTS: A total of 454 lncRNAs with differential m⁶A methylation were identified in GMA-induced 16HBE malignant transformed cells,including 334 which were hypermethylated mRNAs and 120 which were hypomethylated mRNAs (|FC|>2.0,P<0.05). There was a total of 434 differentially expressed mRNAs,among which 236 were up-regulated and 198 were down-regulated (|FC|>2.0,P<0.05). There were 45 m⁶A-methylated mRNAs. The GO analyses showed that SNAP receptor activity,SNARE binding and SNARE complex were the main biological processes. KEGG pathway analyses showed that m⁶A-methylated mRNAs were enriched in SNARE interactions in vesicular transport,non-homologous end-joining,and phenylalanine metabolism. CONCLUSION: Our data indicate that m⁶A methylation played an important role in the process of GMA-induced 16HBE malignant transformation.

Key words: N⁶-methyladenosine, glycidyl methacrylate, high throughput microarray, malignant transformation

中图分类号:

R730.2

王苗, 王全凯, 李昕苇, 马顺鹏, 乌瀚宝栎尔, 许建宁. 甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化相关m⁶A修饰异常mRNA的分析[J]. 癌变·畸变·突变, 2021, 33(6): 405-409.

WANG Miao, WANG Quankai, LI Xinwei, MA Shunpeng, WUHAN Baolier, XU Jianning. m⁶A methylation of mRNAs in glycidyl methacrylate-induced malignant transformation of 16HBE cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 405-409.

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甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化相关m⁶A修饰异常mRNA的分析

m⁶A methylation of mRNAs in glycidyl methacrylate-induced malignant transformation of 16HBE cells

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