CD68巨噬细胞浸润对原发性食管小细胞癌预后的影响

doi:10.3969/j.issn.1004-616x.2022.04.006

癌变·畸变·突变 ›› 2022, Vol. 34 ›› Issue (4): 279-283.doi: 10.3969/j.issn.1004-616x.2022.04.006

• 论著 • 上一篇

CD68巨噬细胞浸润对原发性食管小细胞癌预后的影响

张玉铃¹, 刘迪填², 陈春发³

1. 汕头大学医学院附属肿瘤医院医疗质量管理科, 广东汕头 515041;
2. 汕头大学医学院附属肿瘤医院胸部外科, 广东汕头 515041;
3. 汕头大学医学院附属肿瘤医院乳腺中心, 广东汕头 515041

收稿日期:2022-03-31 修回日期:2022-06-29 发布日期:2022-08-05
通讯作者: 陈春发
作者简介:张玉铃，E-mail：neroli1212@163.com。
基金资助:
汕头市科技计划(190816095262235；汕科府[2019]85)；汕头大学医学院附属肿瘤医院青年科研基金(2020A003)

Impact of CD68 macrophage infiltration on prognosis in primary small cell esophageal carcinoma

ZHANG Yuling¹, LIU Ditian², CHEN Chunfa³

1. Department of Medical Quality Management, Cancer Hospital of Shantou University Medical College, Shantou 515041;
2. Department of Thoracic Surgery, Cancer Hospital of Shantou University Medical College, Shantou 515041;
3. The Breast Cancer Center, Cancer Hospital of Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2022-03-31 Revised:2022-06-29 Published:2022-08-05

摘要/Abstract

摘要： 目的：探讨不同亚型肿瘤相关巨噬细胞在原发性食管小细胞癌(PESC)中的浸润情况及其与患者临床病理指标和预后的关系。方法：回顾性分析汕头大学医学院附属肿瘤医院2000年1月—2019年12月进行手术治疗且临床病理资料完整的69例PESC患者组织标本。采用免疫组织化学法评估CD68巨噬细胞及CD163巨噬细胞在PESC中的浸润情况，卡方检验和Fisher精确检验分析其与PESC临床病理指标的关系，采用Kaplan-Meier生存分析及多因素Cox回归方法分析各检测指标与患者生存预后的关系。结果：CD68及CD163均在巨噬细胞的胞质或胞膜表达。CD68巨噬细胞与肿瘤T分期有关(χ²=6.336，r=-0.303，P=0.012)，与其他各临床病理指标均无明显相关。PESC患者的pTNM分期、手术淋巴结清扫数目、N分期、辅助性化疗及CD68巨噬细胞浸润情况与总生存期有关(P<0.05)。多因素Cox回归分析结果显示pTNM分期、手术淋巴结清扫数目、CD68巨噬细胞是PESC患者独立的预后因素。与CD68巨噬细胞低浸润组相比，CD68巨噬细胞高浸润患者的死亡风险降低[HR=0.340，95% CI (0.179，0.645)，P=0.001]。结论：CD68巨噬细胞高浸润是PESC患者独立的预后影响因素，CD68巨噬细胞高浸润的患者预后明显优于CD68巨噬细胞低浸润的患者。

Abstract: OBJECTIVE: To investigate infiltrations of different subtypes of tumor-associated macrophages in primary esophageal small cell carcinomas (PESC),and their relationships with clinico-pathological parameters and prognosis. METHODS: Clinico-pathological data of PESC from January 2000 to December 2019 in Cancer Hospital of the Shantou University Medical College were analyzed retrospectively. Infiltrations of the CD68 and CD163 macrophages were evaluated by immunohistochemistry. The Chi-square and Fisher's exact tests were used to evaluate their relationships with the clinico-pathological indices. Furthermore,Kaplan-meier and Multivariate Cox regression were used to analyze prognosis for PESC. RESULTS: CD68- and CD163-stained macrophages were located in cytoplasmic or membrane of tumor cells. CD68 macrophage infiltrations,specifically,were correlated with tumor T stages (χ²=6.336,r=-0.303,P=0.012). However,the infiltrations showed no association with the other clinico-pathological variables. Kaplan-meier analyses showed that the pTNM stage, number of surgical lymph node dissection, N stage, adjuvant chemotherapy and infiltration were associated with overall survival (P<0.05). Multivariate Cox regression analyses showed that pTNM stage, the number of lymph node dissection and the infiltration were independent prognostic factors. High infiltrations were significantly associated with overall survival compared with low infiltrations (Hazard ratio=0.340, 95% confidence interval 0.179-0.645; P=0.001). CONCLUSION: High infiltration of CD68 macrophages,but not CD163,was an independent prognostic factor for patients with PESC. Patients with high infiltrations had significantly favorable prognoses than those with low infiltration of CD68 macrophages.

Key words: tumor-associated macrophage, primary esophageal small cell carcinoma, prognosis, CD68, CD163

中图分类号:

R735.1

张玉铃, 刘迪填, 陈春发. CD68巨噬细胞浸润对原发性食管小细胞癌预后的影响[J]. 癌变·畸变·突变, 2022, 34(4): 279-283.

ZHANG Yuling, LIU Ditian, CHEN Chunfa. Impact of CD68 macrophage infiltration on prognosis in primary small cell esophageal carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(4): 279-283.

参考文献

[1] NAGTEGAAL I D, ODZE R D, KLIMSTRA D, et al.The 2019 WHO classification of tumours of the digestive system[J].Histopathology, 2020, 76(2):182-188.
[2] MCKEOWN F.Oat-cell carcinoma of the oesophagus[J].J Pathol Bacteriol, 1952, 64(4):889-891.
[3] FAN N B, WANG Z, HUANG Y H, et al.A retrospective study of 52 patients with primary small cell carcinoma of the esophagus treated with radical surgery[J].Cancer Control, 2021, 28(12):10732748211027147.
[4] YANG C G, WEI C, WANG S Y, et al.Elevated CD163+/CD68+ratio at tumor invasive front is closely associated with aggressive phenotype and poor prognosis in colorectal cancer[J].Int J Biol Sci, 2019, 15(5):984-998.
[5] SICA A, MANTOVANI A.Macrophage plasticity and polarization:in vivo veritas[J].J Clin Invest, 2012, 122(3):787-795.
[6] AMBARUS C A, KRAUSZ S, VAN EIJK M, et al.Systematic validation of specific phenotypic markers for in vitro polarized human macrophages[J].J Immunol Methods, 2012, 375(1/2):196-206.
[7] LIU C Y, LI Y H, YU J Z, et al.Targeting the shift from M1 to M2 macrophages in experimental autoimmune encephalomyelitis mice treated with fasudil[J].PLoS One, 2013, 8(2):e54841.
[8] DONOHOE C L, PHILLIPS A W.Cancer of the esophagus and esophagogastric junction:an 8^th edition staging primer[J].J Thorac Dis, 2017, 9(3):E282-E284.
[9] ZHANG Y L, REN H Z, WANG L, et al.Clinical impact of tumor-infiltrating inflammatory cells in primary small cell esophageal carcinoma[J].Int J Mol Sci, 2014, 15(6):9718-9734.
[10] YAMAMOTO K, MAKINO T, SATO E, et al.Tumorinfiltrating M2 macrophage in pretreatment biopsy sample predicts response to chemotherapy and survival in esophageal cancer[J].Cancer Sci, 2020, 111(4):1103-1112.
[11] GUO F, FENG Y C, ZHAO G, et al.Tumor-associated CD163⁺ M2 macrophage infiltration is highly associated with PD-L1 expression in cervical cancer[J].Cancer Manag Res, 2020, 12:5831-5843.
[12] 汪园圆,朱丹,杜光烨.CD68、CD11c和CD163阳性巨噬细胞在乳腺癌组织中表达及与临床病理特征和预后的关系[J].临床与实验病理学杂志, 2021, 37(12):1486-1489.
[13] NIZET V, JOHNSON R S.Interdependence of hypoxic and innate immune responses[J].Nat Rev Immunol, 2009, 9(9):609-617.
[14] WEBER M, BÜTTNER-HEROLD M, HYCKEL P, et al.Small oral squamous cell carcinomas with nodal lymphogenic metastasis show increased infiltration of M2 polarized macrophages-An immunohistochemical analysis[J].J Cranio Maxillofac Surg, 2014, 42(7):1087-1094.
[15] MINAMI K, HIWATASHI K, UENO S, et al.Prognostic significance of CD68, CD163 and Folate receptor-β positive macrophages in hepatocellular carcinoma[J].Exp Ther Med, 2018, 15(5):4465-4476.
[16] SHABO I, SVANVIK J.Expression of macrophage antigens by tumor cells[J].Adv Exp Med Biol, 2011, 714:141-150.
[17] 李舒眉,冯红超,马竹君,等.CD68⁺和CD163⁺巨噬细胞在口腔鳞状细胞癌中的浸润及作用[J].重庆医学, 2017, 46(20):2764-2766.
[18] 何岸江,胡卫军,李霄,等.CD68⁺、CD163⁺巨噬细胞在结直肠癌组织中的浸润及意义[J].结直肠肛门外科, 2017, 23(4):440-444.
[19] BINGLE L, BROWN N J, LEWIS C E.The role of tumourassociated macrophages in tumour progression:implications for new anticancer therapies[J].J Pathol, 2002, 196(3):254-265.
[20] SUGIMURA K, MIYATA H, TANAKA K, et al.High infiltration of tumor-associated macrophages is associated with a poor response to chemotherapy and poor prognosis of patients undergoing neoadjuvant chemotherapy for esophageal cancer[J].J Surg Oncol, 2015, 111(6):752-759.
[21] 聂尊珍,郭英,陈佳,等.CD47、CD68和CD163在胃癌组织中表达的临床意义[J].山西医科大学学报, 2021, 52(8):956-960.
[22] 杜庆武,庞青松,王平.原发性食管小细胞癌分子机制及治疗进展[J].中国肿瘤临床, 2021, 48(14):738-742.
[23] LI J Y, MA J, WANG H, et al.Population-based analysis of small cell carcinoma of the esophagus using the SEER database[J].J Thorac Dis, 2020, 12(7):3529-3538.

CD68巨噬细胞浸润对原发性食管小细胞癌预后的影响

Impact of CD68 macrophage infiltration on prognosis in primary small cell esophageal carcinoma

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