关键词: 明日叶查尔酮, 酒精性肝损伤, 脂质代谢, 氧化损伤, 细胞凋亡

Abstract: OBJECTIVE： The present study aimed to investigate protective effects of angelica keiskei chaclones (AC) on acute alcohol liver injury in mice. METHODS：Sixty healthy mice were divided randomly into five groups (12 mice per group)： normal control，alcohol model，and low-(5 mg/kg)，medium-(15 mg/ kg) and high-dose (30 mg/kg) groups. AC was intragastrically administrated with a volume of 10 μL/g once a day for 30 days. Mice in the normal control and alcohol model groups were given equal volumes of saline. Twelve hours after the last administration，all mice except those in the normal control group were given 50% alcohol by gavage at a dose of 12 mL/kg. After 12 hours， all mice were sacrificed. Blood samples were collected from eyeballs and serum was isolated. Indicators of liver injury in glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST)， lipid metabolism of low-density lipoprotein cholesterol (LDL-C)、 high-density lipoprotein cholesterol (HDL-C)、 total cholesterol(TC)and triglyceride (TG)， oxidative stress of superoxide dismutase (SOD)、 malondialdehyde (MDA)and glutathione peroxidase (GSH-Px)， and cell apoptosis were evaluated. Liver histopathology was examined as well. RESULTS：Compared with the normal control group， the liver indices in the alcohol control group were significantly increased with obvious inflammatory cell infiltration (P<0.05). Their serum transaminase activity and contents of serum TC， T and LDL-C were obviously increased and HDL was significantly reduced (P<0.05). Their activities of SOD and GSH-Px were significantly decreased， and the content of MDA was increased obviously (P<0.05). Their apoptosis rates of hepatocytes were obviously increased (P<0.05). Compared with the alcohol model group，the liver indices for the medium-and high-dose AC groups were significantly decreased (P<0.05)， and inflammatory cell infiltrations were obviously reduced. Their serum transaminase activities and contents of serum TC， TG and LDL-C were significantly decreased (P<0.05). Their activities of SOD and GSH-Px were effectively increased， and the contents of MDA were reduced obviously (P<0.05). Their apoptosis rates of hepatocytes were also significantly lower than the alcohol model group (P<0.05). CONCLUSION：AC exhibited protective effects on acute alcoholic liver injury. A mechanism may be related to reduction of lipid peroxidation，modulation of lipid metabolism and inhibition of hepatocyte apoptosis.

Key words: angelica keiskei chaclones, alcoholic liver injury, lipid metabolism, oxidative damage, apoptosis