双酚S对秀丽隐杆线虫精子生成相关基因表达的影响

doi:10.3969/j.issn.1004-616x.2023.03.004

癌变·畸变·突变 ›› 2023, Vol. 35 ›› Issue (3): 183-186.doi: 10.3969/j.issn.1004-616x.2023.03.004

• 论著 • 上一篇

双酚S对秀丽隐杆线虫精子生成相关基因表达的影响

唐娇^1,2, 谭剑斌², 茅莉娜³, 张紫虹², 李文立², 胡帅尔², 赵敏²

1. 广东省公共卫生研究院健康风险评估研究室, 广东广州 511430;
2. 广东省疾病预防控制中心卫生毒理所, 广东广州 511430;
3. 广东省生物制品与药物研究所药理研究室, 广东广州 510440

收稿日期:2022-11-03 修回日期:2023-04-17 发布日期:2023-06-03
通讯作者: 赵敏
作者简介:唐娇,E-mail:1075518775@qq.com;谭剑斌,E-mail:tan-sword@163.com。
基金资助:
广东省医学科学技术研究基金(A2019562，C2019055)；广东省中医药局科研项目(20201042，20202026，20212028)

Effects of bisphenol S on expression of spermiogenesis genes in C. elegans

TANG Jiao^1,2, TAN Jianbin², MAO Lina³, ZHANG Zihong², LI Wenli², HU Shuaier², ZHAO Min²

1. Institute of Health Risk Assessment, Guangdong Provincial Institute of Public Health, Guangzhou 511430;
2. Institute of Toxicology, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430;
3. Department of Pharmacological Research, Guangdong Provincial Institute of Biological Products and Materia Medica, Guangzhou 510440, Guangdong, China

Received:2022-11-03 Revised:2023-04-17 Published:2023-06-03

摘要/Abstract

摘要： 目的：探讨双酚S对模式生物秀丽隐杆线虫精子生成相关基因表达的影响。方法：根据双酚S的急性毒性试验结果确定本研究剂量为0、3、30、300 μmol/L，对同步化至L4期的线虫进行24 h暴露，测定其子代数目并用实时荧光定量PCR(qPCR)检测akt-1、swm-1、try-5、spe-4、spe-6基因表达水平。结果：秀丽隐杆线虫于双酚S中暴露24 h后，其半数致死浓度(LC₅₀)为 2 773.672 μmol/L；与对照组相比，暴露后的L4期秀丽隐杆线虫在双酚S剂量为3、30、300 μmol/L时子代数目均显著降低(P<0.05或P<0.01)；akt-1 mRNA在3 μmol/L时的表达水平升高(P<0.05)；swm-1、try-5、spe-4、spe-6 mRNA在30和300 μmol/L时表达水平均升高(P<0.05或P<0.01)。结论：双酚S对秀丽隐杆线虫精子生成相关基因表达有影响。

关键词: 秀丽隐杆线虫, 双酚S, 精子生成, 模式生物

Abstract: OBJECTIVE：To investigate effects of bisphenol S on expression of spermiogenesis genes in Caenorhabditis elegans (C. elegans). METHODS：Nematodes at L4 stage were exposed to bisphenol S for 24 h (with the concentrations of 0，3，30，300 μmol/L based on the acute toxicity test)，brood size and expression levels of akt-1，swm-1，try-5，spe-4 and spe-6 genes were detected by real-time fluorescence quantitative PCR. RESULTS：After the 24 h exposure to bisphenol S，the LC₅₀ was determined to be 2 773.672 μmol/L. Compared to the control group，exposure with the concentrations of 3，30 and 300 μmol/L significantly reduced the brood size (P<0.05 or P<0.01). In addition，the mRNA level of akt-1 was increased at the concentration of 3 μmol/L (P<0.05)；the expression level of swm-1，try-5，spe-4 and spe-6 mRNA were increased in the 30 and 300 μmol/L treatment groups (P<0.05 or P<0.01). CONCLUSION：Expressions of spermiogenesis genes in C. elegans were affected from exposure to bisphenol S.

Key words: Caenorhabditis elegans, bisphenol S, spermiogenesis, model organism

中图分类号:

R994.6

唐娇, 谭剑斌, 茅莉娜, 张紫虹, 李文立, 胡帅尔, 赵敏. 双酚S对秀丽隐杆线虫精子生成相关基因表达的影响[J]. 癌变·畸变·突变, 2023, 35(3): 183-186.

TANG Jiao, TAN Jianbin, MAO Lina, ZHANG Zihong, LI Wenli, HU Shuaier, ZHAO Min. Effects of bisphenol S on expression of spermiogenesis genes in C. elegans[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(3): 183-186.

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双酚S对秀丽隐杆线虫精子生成相关基因表达的影响

Effects of bisphenol S on expression of spermiogenesis genes in C. elegans

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