臭氧急性暴露对大鼠肺组织损伤的影响

doi:10.3969/j.issn.1004-616x.2024.03.008

癌变·畸变·突变 ›› 2024, Vol. 36 ›› Issue (3): 219-223.doi: 10.3969/j.issn.1004-616x.2024.03.008

臭氧急性暴露对大鼠肺组织损伤的影响

孙小宁¹, 苏德奇¹, 杨浩峰², 田亚绒³, 刘慧¹, 黄小溪¹

1. 新疆医科大学公共卫生学院, 新疆特殊环境与健康研究重点实验室, 新疆乌鲁木齐 830017;
2. 乌鲁木齐市疾病预防控制中心, 新疆乌鲁木齐 830023;
3. 延安大学咸阳医院, 陕西咸阳 712099

收稿日期:2023-09-22 修回日期:2024-04-10 出版日期:2024-05-30 发布日期:2024-06-05
通讯作者: 黄小溪
作者简介:孙小宁，E-mail：758515079@qq.com。
基金资助:
新疆维吾尔自治区自然科学基金(2022D01C702)；新疆医科大学医学科学研究所开放课题(YXYJ2023101)；新疆特殊环境与健康重点实验室项目(SKL-SEHR-2021-06)

Effects of acute exposure to ozone on lung tissue injury in rats

SUN Xiaoning¹, SU Deqi¹, YANG Haofeng², TIAN Yarong³, LIU Hui¹, HUANG Xiaoxi¹

1. Xinjiang Key Laboratory of Special Environment and Health Research, School of Public Health, Xinjiang Medical University, Urumqi 830017, Xinjiang;
2. Urumqi Center for Disease Control and Prevention, Urumqi 830023, Xinjiang;
3. Xianyang Hospital, Yan'an University, Xianyang 712099, Shaanxi, China

Received:2023-09-22 Revised:2024-04-10 Online:2024-05-30 Published:2024-06-05

摘要/Abstract

摘要： 目的：建立臭氧急性暴露模型，探究不同浓度臭氧急性暴露对大鼠肺组织损伤的影响。方法：选取SPF级SD大鼠48只，雌雄各半，每组12只，随机分为对照组、1.0、2.0和4.0 ppm组。各组大鼠每天暴露于臭氧环境中4 h，连续暴露7 d，染毒结束后腹主动脉采血并取肺组织。计算大鼠肺系数变化，检测肺组织匀浆中氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)以及血清炎症因子(IL-1β和TNF-α)变化，另进行肺组织病理学观察。结果：与空气对照组相比，4.0 ppm臭氧暴露组雄鼠肺系数升高(P<0.05)。随着臭氧浓度的升高，大鼠肺组织MDA浓度逐渐上升，4.0 ppm组雌鼠、2.0 ppm和4.0 ppm组雄鼠与对照组间的差异均具有统计学意义(P<0.01)；雌鼠肺组织在2.0和4.0 ppm组SOD浓度上升(P<0.01)，雄鼠肺组织中SOD浓度先上升，在2.0 ppm时最高(P<0.01)，再下降，在4.0 ppm时仍高于对照组(P<0.05)；4.0 ppm组雌鼠和各剂量组雄鼠肺组织GSH-Px浓度均升高(P<0.01)；且雌鼠血清中TNF-α浓度升高(P<0.05)，雄鼠血清中IL-1β和TNF-α浓度均升高(P<0.05)。病理组织学观察结果显示，与对照组相比，2.0和4.0 ppm组大鼠肺泡间隔均明显增厚，并出现大面积实变，且雌性大鼠肺实变程度更严重。结论：臭氧会对大鼠肺组织造成病理损伤，导致大鼠肺组织氧化应激反应和炎症反应，且臭氧对肺组织的毒效应具有性别差异。

关键词: 臭氧, 肺损伤, 炎症反应, 急性暴露, 性别差异

Abstract: OBJECTIVE：To develop a rat model for investigating effects of acute ozone exposure on lung tissue injury in rats. METHODS：48 SPF-grade SD rats ，half male and half female，were randomly divided into 4 groups：control，and 1.0，2.0 and 4.0 ppm exposures. The environmental exposure was for 4 h every day for 7 d. After exposure，lung tissues and blood were collected，and changes of lung coefficient were calculated. Oxidative stress indexes of malondialdehyde (MDA)，superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in lung tissue homogenate were measured. Changes of serum inflammatory factors (IL-1β and TNF-α) and pathological status of lung tissues were detected. RESULTS：Compared with the control group，the lung coefficient of rats increased gradually under different concentrations of ozone exposure (P<0.05). In addition，MDA content in lung tissues increased with increased concentrations (P<0.01). SOD content in lung tissue of female rats was increased (P<0.01)，and SOD content in lung tissue of male rats was first increased and then decreased (P<0.05). The content of GSH-Px was increased (P<0.01). The serum levels of TNF-α in female rats were increased (P<0.05)，and the levels of IL-1β and TNF-α in male rats were increased (P<0.05). Histopathological results showed that the alveolar septum of lung tissues in female rats was thickened and there was inflammatory cell infiltration. Thickening of the alveolar septum of the 2.0 and 4.0 ppm groups was worse than that of the control group. In the control group，the pulmonary alveolar septum was slightly thickened and there was mild inflammation. The thickening of alveolar interval in each ozone dose group was more severe than that in the control group，and large-scale lung tissue consolidation was observed in the 2.0 and 4.0 ppm dose groups. CONCLUSION：Ozone exposure caused pathological injury，oxidative stress and inflammation in rat lung tissues，and the toxic effects showed sex gender differences.

Key words: ozone, lung injury, inflammatory response, acute exposure, gender difference

中图分类号:

R114

孙小宁, 苏德奇, 杨浩峰, 田亚绒, 刘慧, 黄小溪. 臭氧急性暴露对大鼠肺组织损伤的影响[J]. 癌变·畸变·突变, 2024, 36(3): 219-223.

SUN Xiaoning, SU Deqi, YANG Haofeng, TIAN Yarong, LIU Hui, HUANG Xiaoxi. Effects of acute exposure to ozone on lung tissue injury in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2024, 36(3): 219-223.

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臭氧急性暴露对大鼠肺组织损伤的影响

Effects of acute exposure to ozone on lung tissue injury in rats

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