新疆哈萨克族食管鳞癌与长链非编码RNA表达的关系

doi:10.3969/j.issn.1004-616x.2020.03.007

癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (3): 194-197,202.doi: 10.3969/j.issn.1004-616x.2020.03.007

新疆哈萨克族食管鳞癌与长链非编码RNA表达的关系

古丽扎热叶·艾库拉¹, 谭遥², 帕力达·阿皮孜阿吉¹

1. 新疆医科大学附属肿瘤医院乳腺放疗科, 新疆乌鲁木齐 830011;
2. 新疆医科大学附属肿瘤医院胸腹放疗科, 新疆乌鲁木齐 830011

收稿日期:2019-08-15 修回日期:2020-05-14 出版日期:2020-05-31 发布日期:2020-06-03
通讯作者: 帕力达·阿皮孜阿吉,E-mail:kpxx9702@163.com E-mail:kpxx9702@163.com
作者简介:古丽扎热叶·艾库拉,E-mail:gulzarya@163.com。
基金资助:
新疆维吾尔自治区自然科学基金（2016D01C354）

Expression of long non-coding RNAs in esophageal squamous cell carcinoma of Kazak nationality in Xinjiang

GULZARIA·Aikula¹, TAN Yao², PALIDA·Apiziaji¹

1. Breast Radiotherapy Department of Xinjiang Medical University Affiliated Cancer Hospital, Urumqi 830011;
2. Thoracoabdominal Radiotherapy Department of Xinjiang Medical University Affiliated Cancer Hospital, Urumqi 830011, Xinjiang, China

Received:2019-08-15 Revised:2020-05-14 Online:2020-05-31 Published:2020-06-03

摘要/Abstract

摘要： 目的：对新疆哈萨克族食管鳞癌组织及癌旁组织长链非编码RNA（lncRNA）表达谱进行分析，并探讨其与食管癌发生和发展的关系。方法：采用基因芯片技术，对4组食管鳞癌及其癌旁组织中差异表达的lncRNA进行筛选，通过lncRNA-mRNA共表达网络对靶基因进行预测，利用GO和KEGG数据库进行富集分析和通路分析，明确lncRNA的功能。结果：癌组织和癌旁组织之间差异表达的lncRNA共318个，与癌旁组织比较，癌组织中193个lncRNA表达上调，125个lncRNA表达下调（P < 0.01）。富集分析表明共有5个GO类别，其中细胞成分有3个，生物过程有1个，分子功能有1个，均通过反式调控方式调控靶基因。通路分析表明这些差异表达的基因可能参与轴突导向信号通路和ECM受体相互作用途径信号通路。结论：新疆哈萨克族食管鳞癌组织中lncRNA的表达水平与癌旁组织有明显差异，差异表达的lncRNA可能参与食管癌的复杂调控网络，并与肿瘤发生和发展有一定的关系。

关键词: 哈萨克族食管鳞癌, 长链非编码RNA, 靶基因预测, 基因芯片

Abstract: OBJECTIVE: To analyze the expression of long non-coding RNA (lncRNA) in esophageal squamous cell carcinoma (ESCC) and adjacent tissues of Kazakh nationality in Xinjiang. METHODS: Differentially expressed lncRNAs in four groups of cancer and a control group were screened by gene chip technology,and the target genes were predicted by lncRNA-mRNA co-expression network. GO and KEGG databases were used for enrichment and Pathway analyses to clarify the function of lncRNA. RESULTS: A total of 318 lncRNAs were differentially expressed between the cancer and control groups. The expression levels of 193 lncRNAs were higher for the cancer than the control groups,and 125 lncRNAs were lower. Enrichment analyses show that there were 5 GO categories,including 3 cellular components,1 biological process and 1 molecular function,all of which were target genes regulated by trans-regulation. Pathway analyses show that these differentially expressed genes might be involved in two signal pathways,such as axonal guidance signal pathway and ECM receptor interaction pathway. CONCLUSION: Expression levels of lncRNAs in Kazakh esophageal squamous cell carcinoma were significantly different from that of the control group. The differentially expressed lncRNAs may be involved in the complex regulatory network of cancer and has a certain relationship with tumorigenesis and progression.

Key words: esophageal squamous cell carcinoma of Kazak nationality, long non-coding RNA, target gene prediction, gene chip

中图分类号:

R730.2

古丽扎热叶·艾库拉, 谭遥, 帕力达·阿皮孜阿吉. 新疆哈萨克族食管鳞癌与长链非编码RNA表达的关系[J]. 癌变·畸变·突变, 2020, 32(3): 194-197,202.

GULZARIA·Aikula, TAN Yao, PALIDA·Apiziaji. Expression of long non-coding RNAs in esophageal squamous cell carcinoma of Kazak nationality in Xinjiang[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 194-197,202.

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新疆哈萨克族食管鳞癌与长链非编码RNA表达的关系

Expression of long non-coding RNAs in esophageal squamous cell carcinoma of Kazak nationality in Xinjiang

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