Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (1): 24-026.doi: 10.3969/j.issn.1004-616x.2009.01.006

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Effect of Hypoxia on Inducing Apoptosis in Non-small Cell Lung Cancer A549 and Expression of Survivin Gene

QIU Rong1, SIMA Jun2, SHEN Xiang-di1, ZHOU Ren3   

  1. 1. Zhejiang Medical College ，Hangzhou 310053 ；2. Red Cross Hospital, Hangzhou 310004； 3.Zhejiang University,Hangzhou 310058，Zhejiang，China
  • Received:2008-09-10 Revised:2008-10-23 Online:2009-01-30 Published:2009-01-30

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Abstract: BACKGROUND AND AIM: To explore the effect of hypoxia on cell growth and apoptosis of non-small cell lung cancer cell line and the change in expression of Survivin gene. MATERIALS AND METHODS: The hypoxia model was set up by using cobalt chloride (CoCl2). MTT assay was used to detect the growth inhibition of A549 in the presence of CoCl2 .Cell morphological transformation was examined with fluorescent microscope. The change of Survivin gene expression was measured by RT-PCR. RESULTS: The growth inhibition rate of A549 effected by CoCl2 was increased in a concentration and time-dependent manner. After exposure to CoCl2 at high concentrations, the Survivin mRNA expression in A549 was down-regulated. CONCLUSION: Hypoxia could inhibit the proliferation of A549 cell and induce apoptosis. The mechanism may be related to the down-regulation of Survivin gene expression.

Key words: hypoxia, non-small cell lung cancer, apoptosis, Survivin