大鼠肺鳞癌癌变过程中基因组甲基化水平动态研究

doi:10.3969/j.issn.1004-616x.2009.01.007

Abstract

Abstract: BACKGROUND AND AIM: To investigate thel changes of genomic methylation during lung carcinogenesis in Wistar rats. MATERIALS AND METHODS: Squamous cell carcinoma of lung was induced with 3-methylcholanthrene (MCA) and diethyinitrosamine (DEN) in iodized oil by left intra-bronchial instillation in 80 Wistar rats. The status of genomic methylation was evaluated by immunohistochemistry with anti 5-mc antibody, and the mean optical density and integrated optical density were measured by image analysis system. RESULTS: A total of 154 specimens of various phases during carcinogenesis were obtained: 25 hyperplasia, 27 squamous metaplasia, 33 dysplasia, 30 carcinoma in situ and 25 infiltrating carcinoma. The immunohistochemical scores of 5-mc were significantly decreased in hyperplasia, squamous metaplasia, dysplasia, carcinoma in situ and infiltrating carcinoma (P<0.01). 5-mc immunostaining was significantly higher in the basal cells than in the luminal cells in normal and carcinogenesis phase (P<0.01). CONCLUSION: The decrease of genomic methylation level suggested that it may be an important epigenetic mechanism during carcinogenesis of lung squamous cell carcinoma in rats.

Key words: lung cancer, DNA methylation, anti 5-mc antibody, carcinogenesis

LIU Wen-bin, LIU Jin-yi, ZHOU Zi-yuan, ZHOU Yan-hong, AO Lin, CUI Zhi-hong, CAO Jia. Genomic Methylation During Carcinogenesis of Lung Squamous Cell Carcinoma in Wistar Rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2009, 21(1): 27-029.

[1]	LIU Xiaoxia, LI Weixia, LI Zhijia, SU Zhenjun, DUAN Xiaohui, HAN Weiwen, WANG Yunxiao, HE Xiaolei, Lu Linlin, JIA Jinhai. Correlations between glutathione peroxidase in serum and clinicopathological outcomes of advanced non-small cell lung cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 286-290,295.
[2]	WANG Xikai, MENG Qinghe, GAO Yanlu. miR-223-3p negatively regulated ECT2 to induce apoptosis in non-small cell lung cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 187-192.
[3]	ZHAO Junwei, HUA Chenfeng, SHANG Pingping, XIE Fuwei, LI Xiang. Induction of oxidative stress by arsenious acid As(Ⅲ) in A549 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 28-31.
[4]	LIU Xiaoxia, LI Weixia, SU Zhenjun, JIA Jinhai, DUAN Xiaohui, LI Zhijia, HE Xiaolei, L Linlin, LIU Rui, WANG Yunxiao. Analyses of clinicopathological characteristics and curative effect of combined radio-with chemo-therapy for advanced non-small cell lung cancer with superoxide dismutase activity in serum [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(5): 350-354.
[5]	ZHANG Fan, LIU Guangchao, ZHANG Zhihan, LIU Fangfang, XIE Qi, GAO Shuqing. Effect of ketogenic diet on growth of subcutaneously transplanted human lung cancer in nude mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 286-291.
[6]	TIAN Xuelei, FENG Jiangbin, TIAN Mei, LIU Qingjie. Use of a methylation chip to detect DNA methylation in human periphery blood lymphocytes exposed to ionizing radiation [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 434-439.
[7]	PENG Changfeng, XIE Xing, KE Yuebin. DNA oxidation and methylation effects in nano-graphene oxide-exposed 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 454-458,463.
[8]	YING Wei, WANG Xingyuan, JIANG Bo, ZHOU Wei. Association between polymorphisms in the miR-146a and susceptibility to non-small cell lung cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(3): 222-226.
[9]	ZHANG Mengying, WANG Boshen, ZHANG Hong, PU Yuepu, YIN Lihong, ZHANG Juan. Effect from 1,4-benzoquinone exposure on DNA methylation in G6PD deficient K562 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(3): 186-192.
[10]	ZHU Jiawei, HU Xiao, WANG Xijun, WANG Yaru, FENG Lin, XIAO Ting. Clinical significance of Skp1 protein expression in non-small cell lung cancer tissues and peripheral blood [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(2): 89-95.
[11]	WU Wen, LI Ping, ZUO Ran, ZENG Qingbo, WU Desheng, XIE Ni, LONG Dingxin, YUAN Jianhui. Correlation between polymorphism of DNA methyltransferase 3B-149C>T and cancer risk:a meta-analysis [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(2): 102-110.
[12]	LI Hui, HU Chengjiu. Significance of expression of MDM2 mRNA and protein in skin scar carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 365-367,373.
[13]	WU Yilan, WANG Han. Inhibition of proliferation by resveratrol in paclitaxel-resistant lung adenocarcinoma cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(3): 200-203,208.
[14]	MA Bangjing, HAO Xianglin, HAN Fei, CHEN Hongqiang, LIU Jinyi, CAO Jia, ZHANG Aihua. Inhibition of proliferation and migration of lung adenocarcinoma cells by SOX30 gene via regulation of DSC3 gene [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(3): 165-170,175.
[15]	PENG Hui, MA Shumei, HONG Weiwei, SUI Jing, ZHANG Yanqiu, LIANG Geyu. Regulatory mechanism of miR-202 on proliferation of A549 lung cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(6): 454-459.

Genomic Methylation During Carcinogenesis of Lung Squamous Cell Carcinoma in Wistar Rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments