Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (1): 48-0051.doi: 10.3969/j.issn.1004-616x.2010.01.012

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Suppression of lymphocyte immune function by MC-LR in vitro

CHEN Hua1;LIN Xiang-ji1;2;WANG Jia-li1;XIE Jian-zhong1;3   

  1. 1. School of Public Health, Fujian Medical University，Fuzhou 350004;2.Xixiang Institute of Public Health Supervision,Shenzhen 518102;3.Fujian Provincial Tumor
  • Received:2009-04-20 Revised:2009-09-27 Online:2010-01-30 Published:2010-01-30
  • Contact: CHEN Hua

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Abstract: OBJECTIVE: To explore the suppression mechanism of MC-LR on lymphocyte immune function in mice. METHODS: Mice splenic lymphocytes were treated with MC-LR at the dose of 0, 1, 3, 5, 10 μg/ml in vitro. The T and B lymphocyte proliferations were examined by MTT assay. The production of IL-2 in cells was measured by ELISA and the apoptosis rate of lymphocytes was deteceted by FCM. RESULTS: The lymphoproliferation was suppressed by MC-LR at all doses. The production of IL-2 was increased at 1 μg/ml but decreased after treatment with MC-LR at 5 and 10 μg/ml. The rate of lymphocyte apoptosis was increased at 10 μg/ml in vitro. CONCLUSION: MC-LR could decrease the production of IL-2 of mouse lymphocyte but increase its apoptosis in vitro. MC-LR could markedly inhibit mice lymphocyte proliferation in vitro.

Key words: microcystin LR, lymphocyte, interleukin-2, immune function

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