Abstract: OBJECTIVE: To investigate the anti-mutation effect of the extracts of pinecones. METHODS：The anti-mutation effect of extracts of pinecones was measured by micronucleus test, with different doses of pinecones alkaline extract solution(50、250 and 500 mg/kg) as samples, cyclophosphamide as the positive group. RESULTS: The mice marrow cells MN rate of high, middle and low doses of extract solution plus cyclophosphamide treatment groups：16‰±0.58‰, 34.75‰±0.85‰ and 40.25‰±0.48‰,respectively. Compared with physiological saline group the difference in MN rate was statistically significant (P<0.01). For extract solution in low dose group compared with CP group, the difference was not statistically significant (P>0.05). In the high and middle dose groups, the difference was statistically significant (P<0.05),inhibition rates were 22.35％ and 65.35％, respectively. Between male and female mices the difference was not statistically significant (P>0.05). CONCLUSION: The extracts of pinecones could suppress mice marrow cells induced by cyclophoshomide, with an obvious dose-response relation. It also showed anti-mutation effects. The extracts of pinecones may have positive effect on tumor prevention.

Key words: The extracts from pinecones, mice, micronuclei, cyclophoshomide, anti-mutation