HSP70多肽复合物的抗肿瘤作用研究

doi:10.3969/j.issn.1004-616x.2013.04.004

Carcinogenesis, Teratogenesis & Mutagenesis

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The anti-tumor effects of HSP70 peptide complex

SHEN Yan-mei¹，SHAN Bao-en²，*，LIU Li-hua²，ZHANG Chao²，ZHOU Kai-xuan²，JIAO Yu-qian²，JIAO Wen-jing³

1. Department of Clinical Laboratory of Shijiazhuang Second Hospital, Shijiazhuang 050011; 2. Research Center, Forth Hospital of Hebei Medical University, Shijiazhuang 050011; 3. Department of Clinical Laboratory of Forth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China

Received:2013-04-02 Revised:2013-06-03 Online:2013-07-30 Published:2013-07-30
Contact: SHAN Bao-en，E-mail：baoenshan@yahoo.com.cn

Abstract

Abstract:

OBJECTIVE: To study on the immunotherapy effects of HSP70 polypeptide complex on colon cancer in mice，and to explore its antitumor mechanism. METHODS：The Colon26 cells were cultured at 42 ℃ for 1 h， then incubated at 37 ℃ for 8 and 12 h，and tumor cell lysates containing high expression of HSP70 polypeptide complex were prepared by repeated freezing and thawing methods. Tumor-bearing mice model was established with Colon26 cells. Tumor cell lysate containing high expression of HSP70 polypeptide complex (test group)，tumor cell lysate containing HSP70 polypeptide complex at 37 ℃ routine culture (control groupⅠ) and PBS (control groupⅡ) were repeatedly injected subcutaneously，in vivo tumorigenicity，growth and survival were monitored.　Expressions of CD3+， CD4+ and CD8+ T cells on the mice splenic monocuclear surface were analyzed by flow cytometry. ELISA was used to assess the production of TGF- β1 and IFN-γ in plasma of the tumor-bearing mice. RESULTS：After tumor-bearing mice were treated with tumor cell lysate containing high expression of HSP70 polypeptide complex，tumor growth，tumor volume and weight were significantly lower than control groupsⅠ and Ⅱ，the survival time was longer (P<0.05). Splenic mononuclear surface molecules of CD8+ T cells was significantly increased in the experimental group compared with the control groupsⅠand Ⅱ (P<0.05). CD3+ and CD4+/CD8+ T cells as well as TGF-β1 significantly reduced (P<0.05) in the plasma in mice of the experimental group compared with the control groupsⅠand Ⅱ，while IFN-γ level increased significantly (P<0.05). CONCLUSION：HSP70 polypeptide complex inhibited the tumor growth significantly，prolonged survival period，indicating that the peptide complex has obvious anti-tumor effects in mice by modulating immunology functions.

Key words: heat shock protein 70, colon cancer, tumor-bearing mice, anti-tumor effect

SHEN Yan-mei，SHAN Bao-en，LIU Li-hua，ZHANG Chao，ZHOU Kai-xuan，JIAO Yu-qian，JIAO Wen-jing. The anti-tumor effects of HSP70 peptide complex[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.04.004.

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The anti-tumor effects of HSP70 peptide complex

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