二溴乙腈对L5178Y细胞的遗传毒性及促凋亡作用

doi:10.3969/j.issn.1004-616x.2015.06.009

Abstract

Abstract: OBJECTIVE: To study the genotoxicity and apoptosis of the mice L5178Y lymphoma cells which was induced by disinfection by-product dibromo acetonitrile in drinking water. METHODS: Cytokinesis-block micronucleus cytome assay(CBMN-cyt) and flow cytometry were used to evaluate genotoxicity and apoptosis in mice L5178Y lymphoma cells treated with different concentrations of dibromo acetonitrile(0.1,1,5 and 10 mol/L). RESULTS: Compared wie negative control group,frequency of micronucleus(MN) of L5178Y lymphoma cells in 1 and 5μmol/L groups increased significantly(P<0.05). The nucleoplasmic bridges(NPBs) in the 1 and 10μmol/L treatment groups and the frequency of nuclear buds(NBUDs) in the 10μmol/L treatment group all increased significantly(P<0.05). However the nuclear divided index(NDI) in the 5 and 10μmol/L treatment groups all decreased significantly(P<0.05). Statistically significant increase of L5178Y lymphoma cell apoptosis were observed in all dibromo acetonitrile treatment groups in comparison to the negative control group(P<0.05). CONCLUSION: Disinfection by-product dibromo acetonitrile in drinking water could obviously induce genotoxicity and apoptosis in L5178Y lymphoma cells.

Key words: dibromo acetonitrile, genotoxicity, apoptosis, cytokinesis-block micronucleus cytome assays, flow cytometry

CLC Number:

R994.6

HONG Liling, WANG Ting, FAN Bin, ZHOU Qingyun, HUANG Yongzhuo, WANG Feng. Genotoxicity and apoptosis in L5178Y cells induced by dibromo acetonitrile[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 450-453,458.

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Genotoxicity and apoptosis in L5178Y cells induced by dibromo acetonitrile

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