原花青素对淀粉样蛋白Aβ25-35诱导的SH-SY5Y细胞毒性的影响

doi:10.3969/j.issn.1004-616x.2017.02.003

Abstract

Abstract:

OBJECTIVE: The influence of proanthocyanidins on oxidative stress,secretion of amyloid protein Aβ_1-42 and soluble amyloid precursor protein sAPPα which were induced by amyloid protein fragment Aβ_25-35 in SH-SY5Y cells was investigated. METHODS: SH-SY5Y cells were treated with Aβ_25-35 of different concentrations (0.1,1.0,10.0 and 20.0 μmol/L) for 24 hours. Cell viability was measured by MTT assay. Cell injury models were built with 1.0 μmol/L Aβ_25-35,with proanthocyanidin of different concentrations added to cell cultures and incubated for 24 h. Concentrations of Aβ_1-42 and sAPPα in cell supernatant were determined by using ELISA. Intracellular content MDA and total SOD were determined by the method of TBA test and WST-8,respectively. RESULTS: Compared with the control group,cells treated with Aβ_25-35 (1 μmol/L) had significantly declined cell viability (P < 0.01),increased Aβ_1-42 level (P < 0.01),declined sAPPα concentration (P < 0.01),increased intracellular MDA content (P < 0.01),and decreased total SOD vitality (P < 0.01). Compared with the Aβ_25-35 injury cell models,proanthocyanidin (0.1,0.5,1.0 and 5.0 μg/mL) significantly improved cell viability (P < 0.05),decreased the secretion of Aβ_1-42 (P < 0.01). Proanthocyanidin (0.5,1.0 and 5.0 μg/mL) increased sAPPα concentration (P < 0.05),decreased MDA content (P < 0.05),and strengthened total SOD vitality (P < 0.05). CONCLUSION: Proanthocyanidin alleviated the Aβ burden and oxidative stress in SH-SY5Y cells which were induced by Aβ_25-35.

Key words: proanthocyanidin, Aβ_1-42, sAPPα, cytotoxicity

CLC Number:

R329.2+8

ZHOU Yapan, ZHANG Xiaoqiang, QU Zhihua, ZHANG Yan, LIANG Xiaoyu. The influences of proanthocyanidin on amyloid beta fragment Aβ_25-35 induced cytotoxicity in SH-SY5Y cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 91-95.

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The influences of proanthocyanidin on amyloid beta fragment Aβ_25-35 induced cytotoxicity in SH-SY5Y cells

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The influences of proanthocyanidin on amyloid beta fragment Aβ25-35 induced cytotoxicity in SH-SY5Y cells

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The influences of proanthocyanidin on amyloid beta fragment Aβ_25-35 induced cytotoxicity in SH-SY5Y cells