Wnt蛋白生成抑制剂2对非小细胞肺癌细胞迁移与侵袭能力的影响

doi:10.3969/j.issn.1004-616x.2017.04.001

Abstract

Abstract:

OBJECTIVE: To investigate the role of the inhibitor of Wnt production 2 (IWP2) on non-small cell lung cancer (NSCLC) cell migration and invasion and to explore mechanisms for the effects. METHODS: H1299 and 95C cell lines were divided into control (cultured with serum-free RPMI-1640 media) and experiment (treated with 10 μmol/L IWP2) groups. Cell migration ability was examined with wound healing and with Transwell assay without Matrigel. Cell invasion ability was determined using Transwell assay with Matrigel. In addition,Western blot was performed to analyze the effect of IWP2 on β-catenin and epithelial-mesenchymal transition(EMT)-related proteins (ZEB1 and Snail). RESULTS: Compared with the control groups,the wound healing results demonstrated that cells cultured with IWP2 had decreased wound closure both in H1299 and 95C (P < 0.01). Transwell assay without Matrigel showed that fewer cells migrated to the lower chambers after being treated with IWP2 (P < 0.01). The Transwell assay with Matrigel indicated that cell invasion ability was also significantly decreased in the experimental compared with the control groups. Besides,after being treated with IWP2,expression of β-catenin was obviously reduced (41.3% in H1299 and 36.1% in 95C). In addition,IWP2 also decreased the expression of ZEB1 (51.8% in H1299 and 40.9% in 95C) and Snail (43.2% in H1299 and 30.7% in 95C). CONCLUSION: Our study revealed that IWP2 down-regulated EMT via blocking β-catenin which led to the inhibition of NSCLC cell migration and invasion.

Key words: the inhibitor of Wnt production 2, non-small cell lung cancer, migration, invasion, β-catenin

CLC Number:

R734.2

ZHANG Jing, TENG Yu, ZHAO Xiaoting, JIANG Mei, YUE Wentao. Effects of the inhibitor of Wnt production 2 on cell migration and invasion in non-small cell lung cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(4): 245-250.

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Effects of the inhibitor of Wnt production 2 on cell migration and invasion in non-small cell lung cancer

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