烟气暴露30天对大鼠心脏基因表达谱的影响

doi:10.3969/j.issn.1004-616x.2021.04.009

Abstract

Abstract: OBJECTIVE: To study effects of smoke exposure on gene expression in hearts of rats using gene chip technology. METHODS: Six male SD rats were divided into two groups:control (n=3) and smoke exposure (n=3). The exposure group were exposed to mainstream smoke for 30 days. Rat hearts were removed and differentially expressed genes were screened using gene chip technology. Differentially-expressed genes were analyzed using gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) databases. RESULTS: Compared with the control group,86 genes with differential expression of more than 2 times were screened out in the smoke exposure group,among which 60 genes were up-regulated and 26 genes were down-regulated. Differentially expressed genes were enriched in 292 biological processes:72 cellular components,96 molecular functions and 61 signaling pathways. These processes were involved in mitogen-activated protein kinase binding,alpha-beta T cell receptor complex,positive regulation of cellular extravasation,and tyrosine metabolism. CONCLUSION: After exposure to smoke for 30 days,differentially expressed genes and signaling pathways in the hearts of rats were mainly involved in molecular binding,cell cycle control and signal transduction,etc.

Key words: smoke exposure, rat heart, gene, bioinformatics

CLC Number:

R994.6

LI Mei, SU Jiakun, YIN Jingjing, XU Da, QIN Xiujun, AN Quan, CAI Jibao. Effects of 30-day smoke exposure on gene expression profile in hearts of rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 291-295.

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Effects of 30-day smoke exposure on gene expression profile in hearts of rats

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