双酚S对秀丽隐杆线虫精子生成相关基因表达的影响

doi:10.3969/j.issn.1004-616x.2023.03.004

Abstract

Abstract: OBJECTIVE：To investigate effects of bisphenol S on expression of spermiogenesis genes in Caenorhabditis elegans (C. elegans). METHODS：Nematodes at L4 stage were exposed to bisphenol S for 24 h (with the concentrations of 0，3，30，300 μmol/L based on the acute toxicity test)，brood size and expression levels of akt-1，swm-1，try-5，spe-4 and spe-6 genes were detected by real-time fluorescence quantitative PCR. RESULTS：After the 24 h exposure to bisphenol S，the LC₅₀ was determined to be 2 773.672 μmol/L. Compared to the control group，exposure with the concentrations of 3，30 and 300 μmol/L significantly reduced the brood size (P<0.05 or P<0.01). In addition，the mRNA level of akt-1 was increased at the concentration of 3 μmol/L (P<0.05)；the expression level of swm-1，try-5，spe-4 and spe-6 mRNA were increased in the 30 and 300 μmol/L treatment groups (P<0.05 or P<0.01). CONCLUSION：Expressions of spermiogenesis genes in C. elegans were affected from exposure to bisphenol S.

Key words: Caenorhabditis elegans, bisphenol S, spermiogenesis, model organism

CLC Number:

R994.6

TANG Jiao, TAN Jianbin, MAO Lina, ZHANG Zihong, LI Wenli, HU Shuaier, ZHAO Min. Effects of bisphenol S on expression of spermiogenesis genes in C. elegans[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(3): 183-186.

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Effects of bisphenol S on expression of spermiogenesis genes in C. elegans

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