腺苷酸活化蛋白激酶在哈萨克族食管鳞癌组织中的表达及意义

doi:10.3969/j.issn.1004-616x.2023.04.007

Abstract

Abstract: OBJECTIVE： To investigate expression levels of the AMPK in Kazakh esophageal squamous cell carcinoma，its role in lipid metabolism reprogramming，and its relationship with clinicopathological features and prognosis. METHODS： Sixty-five Kazakh patients with esophageal squamous cell carcinomas were selected. AMPK mRNA expression levels in the cancer cells and the adjacent normal tissues were detected using qPCR. AMPK protein expressions were detected using immunohistochemistry. Kaplan-Meier method was used to draw the survival curve，and to analyze the correlation between AMPK expression and the prognosis of the carcinomas. Cox proportional hazards regression analysis was used to analyze the risk factors affecting the poor prognosis of the cancer patients. AMPK was stably knocked down in the esophageal cancer cell line，KYSE150，and lipid qualitative and quantitative analyses were performed using ultra performance liquid chromatography-mass spectrometry. RESULTS： The mRNA levels and protein positive rates of AMPK in the carcinoma tissues were significantly higher than those in adjacent tissues (P<0.05). The expression level of AMPK was correlated with the degree of differentiation，lymph node metastasis and TNM stage (P<0.05). The expression levels of AMPK were correlated with the degrees of differentiation，lymph node metastasis and TNM stages (P<0.05). The positive expression of AMPK was a reliable risk factor for poor prognosis of esophageal squamous cell carcinoma among Kazakhs. Through the absolute quantitative analysis of lipid content in esophageal cancer cells，it was found that the lipid content and its changing trend were different in different lipid grades. A total of 10 lipid subclasses were identified，consisting of fatty acids (7.88%)，glycerides (60.77%)，glycerides sphingolipids (21.35%)，sphingolipids (9.62%) and sterol lipids (0.38%). AMPK induced significant changes in lipid metabolism in esophageal cancer cells. A total of 76 differential lipid metabolites were found，among which 37 were up-regulated and 29 were down-regulated. The up-regulation of triglyceride (TAG) and the down-regulation of sphingolipid (SM)，diglyceride (DAG) and phosphatidyl ethanolamine (PE) were observed. Targeted lipid quantitative analysis results suggest that AMPK might be involved in lipid metabolic reprogramming in Kazakh patients with esophageal squamous cell carcinoma. CONCLUSION： The mRNA level and protein positive rate of AMPK in esophageal squamous cell carcinoma tissues were significantly higher than those in adjacent tissues (P<0.05). The expression level of AMPK was correlated with the degree of differentiation，lymph node metastasis and TNM stage (P<0.05). The positive expression of AMPK was a reliable risk factor for poor prognosis of Kazakh esophageal squamous cell carcinoma.

Key words: esophageal squamous cell carcinoma, Kazakh, AMPK, survival prognosis, metabolic reprogramming

CLC Number:

R735.1

LIU Ruixue, ZHANG Liwei. Expression and significance of AMPK esophageal squamous cell carcinoma among Kazakhs[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(4): 285-291.

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Expression and significance of AMPK esophageal squamous cell carcinoma among Kazakhs

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