Carcinogenesis, Teratogenesis & Mutagenesis ›› 2024, Vol. 36 ›› Issue (2): 143-149.doi: 10.3969/j.issn.1004-616x.2024.02.011

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Protective effect of aqueous extracts from Phyllanthus emblica on longevity and climbing ability of Drosophila melanogaster

LI Lingzhi1, HU Xueqin1, HUANG Yanan1, JIA Yizhen1, WANG Xu1,2, GUO Xihan1   

  1. 1. College of Life Sciences, Yunnan Normal University, Engineering Research Center for Sustainable Development and Utilization of Bioenergy, Ministry of Education, Kunming 650500, Yunnan;
    2. Taizhou Yeda Institute of Gene and Cell Therapy, Taizhou 318000, Zhejiang, China
  • Received:2023-11-05 Revised:2023-12-25 Published:2024-04-11

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Abstract: OBJECTIVE： phyllanthus emblica (PE) is a medicinal and food plant rich in a variety of phenolic substances and organic acids. In this study，we investigated the effect of PE aqueous extract on the lifespan and climbing ability of Drosophila melanogaster (D.melanogaster) under normal physiological and Cadmium (Cd) stress conditions. METHODS： PE aqueous extracts (1.6， 8 and 16 mg/mL) were applied to wild-type male and female D.melanogaster to find the optimal concentrations and treatment times to investigate their effects on lifespan and climbing ability，and on the SOD，CAT，and MDA. Then 5，10，20，40 mg/L CdCl2 was applied to both male and female D.melanogaster to find the optimal concentration that effectively reduced the lifespan of D.melanogaster. Then，the optimal concentration of CdCl2 and PE extracts were mixed and used to treat both male and female D.melanogaster and to explore the effects on D.melanogaster under Cd stress. RESULTS：Compared with the control，16 mg/mL PE aqueous extract extended the lifespan of male and female D.melanogaster under normal physiological conditions (P<0.05). Under the optimal concentrations of PE (1.6 mg/mL for females and 8 mg/mL for males)， the climbing abilities of both were improved and showed gender differences，with the optimal time for females of 22 d and males of 10 d，but the optimal concentration of PE aqueous extracts did not increase the antioxidant enzyme activities in males and females for 10，22，33and 45 d，respectively. From treatment with 40 mg/L CdCl2，the average lifespans for both were reduced (P<0.01) compared with the control. Even with treatment from 40 mg/L CdCl2，8 and 16 mg/mL PE helped to prolong the lifespan of males but their climbing ability was significantly reduced after 18 d of treatment (P<0.05)， while the treatments of 1.6， 8 and 16 mg/mL PE did not generate any significant effects on the lifespan and climbing ability of females (P>0.05). CONCLUSION：PE aqueous extract prolonged the lifespan and climbing ability of both male and female D.melanogaster under normal physiological conditions，and prolonged the lifespan of males under Cd stress，but reduced their climbing ability. In addition，there was a gender difference in the protective effect of PE on D.melanogaster.

Key words: Phyllanthus emblica Linn, Cadmium stress, longevity, climbing ability, Drosophila melanogaster

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