白细胞介素-17过表达胃癌细胞在小鼠体内的成瘤效应研究

doi:10.3969/j.issn.1004-616x.2019.04.006

Abstract

Abstract: OBJECTIVE:The aim was to investigate tumorigenic effects of interleukin-17 (IL-17) overexpressing gastric cancer cells in mice. METHODS:MFC,MFC/pcDNA3.1 and MFC/IL-17 cells were inoculated subcutaneously into 615 mice,with 10 mice in each group. Three weeks after the inoculation when tumors became obvious,mRNA and protein expressions of IL-17,vascular endothelial growth factor (VEGF) and Podoplanin in tumor tissues were detected using RT-PCR and Western-blots. Micro-vessel density was detected by immunohistochemistry. RESULTS:Tumor nodules developed on 5-7 d after inoculation and grew rapidly in mice inoculated with MFC/IL-17 (MFC/IL-17 mice). The average tumor weights in MFC/IL-17 mice[(5.384±0.391) g] were significantly more than that in MFC mice[(1.726±0.445) g] and MFC/pcDNA3.1 mice[(2.064±0.397) g] (P < 0.05). In addition,the mRNA and protein expressions of IL-17,VEGF and Podoplanin in MFC/IL-17 mice were significantly higher than that in MFC and MFC/pcDNA3.1 mice (P < 0.05). The micro-vessel densities were significantly increased in MFC/IL-17 mice than that in MFC and MFC/pcDNA3.1 mice (P < 0.05). CONCLUSION:Our data show that expression of IL-17 accelerated tumor growth in vivo by up-regulating the expression of VEGF,Podoplanin and CD31,thus promoting angiogenesis and lymphatic vessel formation.

Key words: IL-17, gastric cancer cells, angiogenesis, tumor-promoting effect

CLC Number:

R735.2

ZHANG Zhiping, SHAN Baoen, SONG Jingjing, WANG Hao, LIU Yingzi, CHEN Wei, GAO Liping, LI Qiaoxia. Tumorigenic effect of interleukin?17 overexpressing gastric cells in mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 295-299.

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Tumorigenic effect of interleukin?17 overexpressing gastric cells in mice

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