癌变·畸变·突变 ›› 2009, Vol. 21 ›› Issue (3): 206-209.doi: 10.3969/j.issn.1004-616x.2009.03.012

• 论著 • 上一篇    下一篇

吲哚-3-甲醇诱导人鼻咽癌细胞凋亡作用及机制的初步研究

朱　伟1, 魏　青2, 杨光宇1, 杨杏芬3,   

  1. 1.广州市疾病预防控制中心毒理科，广州　510080；2.中山大学公共卫生学院预防
  • 收稿日期:2008-11-14 修回日期:2009-01-06 出版日期:2009-05-30 发布日期:2009-05-30

Pilot Study on the Mechanism of CNE Cell Apoptosis Induced by Indole-3-carbinol

ZHU Wei1, WEI Qing2, YANG Guang-yu1, YANG Xing-fen3,   

  1. 1. Department of Toxicology, Guangzhou Center for Disease Control and Prevention, Guangzhou 510080；2.Department of Preventive Medicine, School of Public Health, Zhongshan University, Guangzhou 510080;3. Guangdong Center for Disease Control and Prevention, Guangzhou 510300,China
  • Received:2008-11-14 Revised:2009-01-06 Online:2009-05-30 Published:2009-05-30

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摘要： 背景与目的： 了解吲哚-3-甲醇在体外对人鼻咽癌CNE细胞增殖的影响及其诱导CNE细胞凋亡的作用及其可能机制。 材料与方法： 采用WST-1法检测吲哚-3-甲醇在体外对人鼻咽癌CNE细胞增殖的抑制作用；用Hochest33258荧光染色法及TUNEL法观察吲哚-3-甲醇诱导CNE细胞的凋亡；用Western blotting方法检测凋亡相关信号分caspase-9、caspase-3蛋白表达情况，实验同时设空白对照组及溶剂对照组。 结果： 吲哚-3-甲醇在体外可以明显抑制人鼻咽癌细胞增殖，诱导细胞凋亡并呈剂量依赖性，且在细胞凋亡过程中，半胱天冬酶家族成员caspase-9和caspase-3 活化片段表达阳性。 结论： 吲哚-3-甲醇在体外可抑制人鼻咽癌CNE细胞增殖并诱导其发生凋亡，且凋亡的发生可能与caspase-9、caspase-3活化有关。

关键词: 吲哚-3-甲醇, 人鼻咽癌细胞, caspase-9, caspase-3

Abstract: BACKGROUND AND AIM: To study the effect of indole-3-carbinol(I3C) in CNE cells as well as the underlying mechanisms. MATERIALS AND METHODS: The inhibition of cell proliferation was assayed by WST-1 agent, and the apoptosis was shown with Hochest 33258 and TUNEL staining. To study the mechanism of apoptosis, the expression of the cleaved caspase-9 and the cleaved caspase-3 were detected with western immublotting assay. RESULTS: I3C could inhibit the proliferation of CNE cells and apoptosis could be induced by I3C in a dose-dependent manner. Furthermore, the expressions of cleaved caspase-9 and cleaved caspase-3 appeared to be increased significantly as compared with those of the control. CONCLUSION: I3C could induce apoptosis of CNE cells dose-dependently, and caspase-9 and caspase-3 pathways might be involved.

Key words: indole-3-carbinol, CNE cell, caspase-9, caspase-3

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