癌变·畸变·突变 ›› 1998, Vol. 10 ›› Issue (1): 11-015.doi: 10.3969/j.issn.1004-616x.1998.01.005

• 论著 • 上一篇    下一篇

环氧乙烷的遗传毒性研究　Ⅷ小鼠肝细胞DNA 定量分析研究

王金茂1 　张树人2 　陆静芬2 　钟　坤2 　张　龄2 ②   

  1. 1 浙江医科大学　2 上海职工医学院　200237
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-01-30 发布日期:1998-01-30

STUDY ON THE GENOTOXICITY OF ETHYLENE OXIDE Ⅷ STUDY OF DNA QUANTITATIVE ANALYSIS IN THE MOUSE HEPATOCYTES

Wang J inmao1 ,Zhang Shuren2 ,Lu J ingfen2 ,Zhong Kun2 ,Zhang Ling2 ②   

  1. 1 Zhejiang Medical University , 2 S hanghai medical College f or Heal th S taf f , S hanghai 200237
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-01-30 Published:1998-01-30

PDF

2536

摘要： 本研究应用IBA S 显微数字图象分析系统,对不同剂量环氧乙烷( ethylene oxide , EtO) 染毒小鼠的肝细胞核DNA 含量进行测定的结果显示:染毒小鼠肝细胞核DNA 的含量均较对照组增高。特别引起注意的是雄性染毒小鼠肝细胞核DNA 的含量明显高于雌性,其中雄性染毒组与对照组、雄性染毒组与雌性染毒组之间,肝细胞核DNA 含量的平均值比较,均有显著差异,经K —S 检验结果P < 0. 01。据此可以提示:环氧乙烷不但可以引起实验小鼠肝细胞DNA 的复制,诱发肝细胞抵近恶性变的倾向,而且雄性小鼠肝细胞对EtO的敏感性明显高于雌性。

关键词: 环氧乙烷, 肝细胞, DNA 定量分析

Abstract: With the digital image analytic system ,this study is to quantitate the DNA in hepatocytes nuclei of exposed mice with different doses of ethylene oxide. The result s showed that the quantity of DNA in the hepatocytes nuclei of exposed groups is higher than that in the cont rol roup. It is worth noticing ,the DNA quantity in male groups is obviously higher than that in the females.By comparing the male exposed groups with cont rol group and the male exposed groups with female exposed groups ,their DNA quantity in the nuclei of hepatocytes are both significant different . The K- S test P < 0. 01. The data suggested that EtO not only caused the duplication of DNA in the experimental mice hepatocytes but also induced the tendency of approching malignant changes in the hepatocytes. The hepatocytes in the male mice are more sensitive to EtO than those in the female.

Key words: ethylene oxide, hepatocyte, quantitative analysis of DNA

版权所有 ©2019 《癌变·畸变·突变》编辑部 E-mail： office@egh.net.cn
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持：support@magtech.com.cn
粤ICP备 10216025号