Cyclin D1 蛋白在碳酸锂对抗黄曲霉素B1 诱导大鼠肝癌过程中的表达及意义

doi:10.3969/j.issn.1004-616x.2000.02.006

癌变·畸变·突变 ›› 2000, Vol. 12 ›› Issue (2): 82-083.doi: 10.3969/j.issn.1004-616x.2000.02.006

Cyclin D1 蛋白在碳酸锂对抗黄曲霉素B1 诱导大鼠肝癌过程中的表达及意义

杨光红,张爱华,洪　峰,陆　爽

贵阳医学院预防医学系,贵州　贵阳　550004

收稿日期:1999-08-03 修回日期:1999-11-27 出版日期:2000-04-30 发布日期:2000-04-30

THE EXPRESSION OF CYCLIN D1 PROTEIN OF LITHIUM CARBONATE ON THE DEVELOPMENT OF RAT HEPATIC CARCINOMA INDUCED BY AFLATOXIN B1 AND ITS SIGNIFICANCE

YANG Guang - hong , ZHANG Ai - hua , HONG Feng , LU Shuang

Department of Prevetive Medicine , Guiyang Medical College , Guiyang 　550004 , China

Received:1999-08-03 Revised:1999-11-27 Online:2000-04-30 Published:2000-04-30

摘要/Abstract

摘要： 目的与方法:应用免疫组化法检测碳酸锂(Li2CO3) 对抗黄曲霉素B1 (AFB1) 诱导大鼠肝癌过程中癌基因蛋白cyclin D1的表达及意义。结果:cyclin D1 在诱癌早期(实验第6 周) 即有表达,第10 周显著增高,差异均有显著性( P < 0. 01) 。阳性对照组(B) 组cyclin D1 蛋白阳性率最高,碳酸锂同时给药组(C 组) 及先期给药组(D 组) 阳性率显著降低,C 组阳性率略高于D组。结论:Li2CO3 可明显对抗AFB1 诱导肝癌过程中cyclin D1 的表达,通过抑制细胞的持续性增殖从而起到抗癌作用。cyclinD1 的免疫组化检查有助于肿瘤的早期发现和追踪观察。

关键词: cyclin D1, 碳酸锂, 黄曲霉毒素, 大鼠肝癌

Abstract: Purpose and Methods : Using immunohisthocemical assay , the expression of cyclin D1 protein of lithium carbonate on the development of rat hepatic carcinoma induced by aflatoxin B1 and it s significance were studied. Results : The expression of cyclin D1 occurred in the early stage (the 6th week) and significantly increased in the 10th week. The positive rate of positive cont rol group (B) was the highest and that of group t reated with Li2CO3 simultaneously (C) and group pre2t reated with Li2CO3 . (D) significantly decreased , but group C > group D. Conclusion : It suggested that Li2CO3 has the effect on anti2expression of cyclin D1 in aflatoxin B1 induced rat hepatic carcinoma , and can rest rain cell continuous roliferation , so it may be anticarcinogenic ; and that the immunohistochemical assay of cyclin D1 may help early diagnosis and monitoring.

Key words: cyclin D1, Li2CO3, AFB1, rat hepatic carcinoma

中图分类号:

R730.231

杨光红, 张爱华, 洪　峰, 陆　爽. Cyclin D1 蛋白在碳酸锂对抗黄曲霉素B1 诱导大鼠肝癌过程中的表达及意义[J]. 癌变·畸变·突变, 2000, 12(2): 82-083.

YANG Guang - hong , ZHANG Ai - hua , HONG Feng , LU Shuang. THE EXPRESSION OF CYCLIN D1 PROTEIN OF LITHIUM CARBONATE ON THE DEVELOPMENT OF RAT HEPATIC CARCINOMA INDUCED BY AFLATOXIN B1 AND ITS SIGNIFICANCE[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2000, 12(2): 82-083.

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Cyclin D1 蛋白在碳酸锂对抗黄曲霉素B1 诱导大鼠肝癌过程中的表达及意义

THE EXPRESSION OF CYCLIN D1 PROTEIN OF LITHIUM CARBONATE ON THE DEVELOPMENT OF RAT HEPATIC CARCINOMA INDUCED BY AFLATOXIN B1 AND ITS SIGNIFICANCE

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