胚胎早期低剂量甲基汞暴露对大鼠的行为致畸效应

doi:10.3969/j.issn.1004-616x.2001.03.003

癌变·畸变·突变 ›› 2001, Vol. 13 ›› Issue (3): 143-146.doi: 10.3969/j.issn.1004-616x.2001.03.003

胚胎早期低剂量甲基汞暴露对大鼠的行为致畸效应

刘　苹, 吴锡南, 纳冬荃, 李　健, 毛玉明, 刘　毅

昆明医学院预防医学系环境卫生学教研室,云南　昆明　650031

收稿日期:2000-12-20 修回日期:2001-02-26 出版日期:2001-07-30 发布日期:2001-07-30

NEUROBEHAVIORAL TERATOGENIC EFFECT ON RAT OFFSPRING AFTER EARLY PREGNANT EXPOSURE TO METHYLMERCURY ATLOW DOSE

LIU Ping, WU Xi-nan, NA DOng-quan, etal .

Department of Environmental Health, Faculty of Public Health, Kunming Medical College, Kunming 650031, China

Received:2000-12-20 Revised:2001-02-26 Online:2001-07-30 Published:2001-07-30

摘要/Abstract

摘要： 目的:探讨胚胎早期甲基汞低剂量暴露对大鼠仔代的行为致畸效应。方法:Wistar 大鼠雌性80 只、雄性20 只以3 :1 交配,大鼠随机分为4 组,于妊娠6～9 d 用氯化甲基汞0. 00 mg/ (kg·bw·d) 、0. 01 mg/ (kg·bw·d) 、0. 05 mg/ (kg·bw·d) 、2. 00 mg/(kg·bw·d) 灌胃染毒。进行胚胎毒性研究;记录201 只仔鼠出生后早期生理发育和神经行为发育指标;10 周龄的仔鼠32 只进行操作行为测试;24 只进行脑组织形态学检查和单胺类神经递质(去甲肾上腺素、多巴胺、52羟色胺) 测定(荧光分光光度法) 。结果:胚胎早期低剂量甲基汞对胎仔体重及尾长发育有抑制作用( P < 0. 01) ,暴露组仔鼠的体重增长、早期生理发育及神经行为发育滞后于对照组( P < 0. 05 或P < 0. 01) ;操作行为成绩均比对照组降低( P < 0. 05 或P < 0. 01) ;3 个暴露组仔鼠均未观察到脑组织形态学改变,但单胺类神经递质含量均比对照组显著增高( P < 0. 05 或P < 0. 01) 。所有结果呈现出剂量2反应关系。结论胚胎早期低剂量甲基汞暴露有一定的胚胎毒性,可影响仔鼠神经系统的发育,导致行为改变。

关键词: 行为致畸, 仔代, 甲基汞, 大鼠

Abstract: Purpose : To detect the neurobehavioral teratogenic effect of methylmercury exposure at early embryonic stage on off spring. Methods : Methylmercury chloride was force2fed to the Wistar rat s during the 6th～9th day after conception at doses of 0. 00 mg/ (kg·bw·d) , 0. 01 mg/ (kg·bw·d) , 0. 05 mg/ (kg·bw·d) and 2. 00mg/ (kg·bw·d) . The toxicity in the embryos , developmental landmarks of 201 off spring and operant behavior test of 32 off spring were measured. The brain morphology and the levels of monamine neurot ransmitters (NA ,DA , 52HT) were tested in 24 off spring at 10 weeks old. Double blind was used in whole experiment . Results :In 3 exposure groups , the body weight and tail development of embryos were slower ( P < 0. 01) , the early developmental landmarks and neurobehavioral development of off spring were delayed ( P < 0. 05) , and their performances in operant behavior were worse than those of the cont rol group ( P < 0. 05) . No histomorphological alteration in the off spring brains was observed in all groups. The levels of monamine neurot ransmitters in brains of 3 exposure groups were increased ( P < 0. 05) . Some result s showed a dose2effect relationship. Conclusion : Early pregnant exposure to methylmercury at a low dose showed some embryotoxicity and adverse effect s on the developing nervous system of the rat off spring and also resulted in neurobehavioral changes.

Key words: methylmercury, behavioral teratogenicity, off spring, Wistar rat

中图分类号:

刘　苹, 吴锡南, 纳冬荃, 李　健, 毛玉明, 刘　毅. 胚胎早期低剂量甲基汞暴露对大鼠的行为致畸效应[J]. 癌变·畸变·突变, 2001, 13(3): 143-146.

LIU Ping, WU Xi-nan, NA DOng-quan, etal .. NEUROBEHAVIORAL TERATOGENIC EFFECT ON RAT OFFSPRING AFTER EARLY PREGNANT EXPOSURE TO METHYLMERCURY ATLOW DOSE[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2001, 13(3): 143-146.

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胚胎早期低剂量甲基汞暴露对大鼠的行为致畸效应

NEUROBEHAVIORAL TERATOGENIC EFFECT ON RAT OFFSPRING AFTER EARLY PREGNANT EXPOSURE TO METHYLMERCURY ATLOW DOSE

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